ENZYME-LINKED-IMMUNOSORBENT-ASSAY COMPARED WITH GAS-CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE DETERMINATION OF TRIAZINE HERBICIDES IN WATER

被引:286
作者
THURMAN, EM
MEYER, M
POMES, M
PERRY, CA
SCHWAB, AP
机构
[1] U.S. Geological Survey, Water Resources Division, Lawrence, Kansas 66049
[2] Department of Agronomy, Kansas State University, Manhattan
关键词
D O I
10.1021/ac00217a027
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An enzyme-linked Immunosorbent assay (ELISA) was compared to a gas chromatography/mass spectrometry (GC/MS) procedure for the analysis of triazine herbicides and their metabolites In surface water and groundwater. Apparent recoveries from natural water and spiked water by both methods were comparable at 0.2-2 µg/L. Solid-phase extraction (SPE) was examined also, and recoveries were determined for a suite of triazine herbicides. A significant correlation was obtained between the ELISA and GC/MS method for natural water samples that were extracted by SPE. Because ELISA was developed with an atrazlne-llke compound as the hapten with conjugation at the 2-position, It was selective for triazines that contained both ethyl and Isopropyl side chains. Concentrations for 50% Inhibition (ICgg) were as follows: atrazlne, 0.4 µ/L; ametryne, 0.45 µ/L; prometryn and propazlne, 0.5 µ/L; prometen, 0.7 µ/L; slmazlne and terbutryn, 2.5 µ/L; hydroxyatrazlne, 28 µ/; deethylatrazlne and delsopropylatrazlne, 30 µ/L; cyanazlne, 40 µ/L; dkfealkylatrazlne had no response. The combination of screening analysis by ELISA, which requires no sample preparation and works on 180 µof sample, and confirmation by GC/MS was designed for rapid, Inexpensive analysis of triazine herbicides In water. © 1990, American Chemical Society. All rights reserved.
引用
收藏
页码:2043 / 2048
页数:6
相关论文
共 27 条
[1]  
[Anonymous], 1980, ANAL CHEM, V52, P2242
[2]   SOLID-PHASE EXTRACTION AND CAPILLARY GAS-CHROMATOGRAPHIC DETERMINATION OF TRIAZINE HERBICIDES IN WATER [J].
BARDALAYE, PC ;
WHEELER, WB .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1986, 25 (1-3) :105-113
[3]   DETERMINATION OF ATRAZINE RESIDUES IN WATER AND SOIL BY ENZYME-IMMUNOASSAY [J].
BUSHWAY, RJ ;
PERKINS, B ;
SAVAGE, SA ;
LEKOUSI, SJ ;
FERGUSON, BS .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1988, 40 (05) :647-654
[4]   DETERMINATION OF ATRAZINE RESIDUES IN FOOD BY ENZYME-IMMUNOASSAY [J].
BUSHWAY, RJ ;
PERKINS, B ;
SAVAGE, SA ;
LEKOUSI, SL ;
FERGUSON, BS .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1989, 42 (06) :899-904
[5]   ANALYSIS OF ATRAZINEIN UNDERGROUND WATERS AT PART PER TRILLION LEVELS AS AN EARLY WARNING METHOD FOR CONTAMINATION AND FOR SOIL DISTRIBUTION STUDIES [J].
DAVOLI, E ;
BENFENATI, E ;
BAGNATI, R ;
FANELLI, R .
CHEMOSPHERE, 1987, 16 (07) :1425-1430
[6]   RAPID-DETERMINATION OF SIMAZINE AND ATRAZINE IN WATER AT THE PARTS-PER-TRILLION (1012) LEVEL - EXTRACTION BY A MINIATURIZED CARBOPACK-B TRAP FOLLOWED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
DICORCIA, A ;
MARCHETTI, M ;
SAMPERI, R .
JOURNAL OF CHROMATOGRAPHY, 1987, 405 :357-363
[7]   A RAPID AND SENSITIVE HPLC PROCEDURE FOR THE DETERMINATION OF ATRAZINE RESIDUES IN SOIL-WATER EXTRACTS [J].
FERRIS, IG ;
HAIGH, BM .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1987, 25 (04) :170-179
[8]  
HUANG LQ, 1989, J ASSOC OFF ANA CHEM, V72, P349
[9]  
HUBER SJ, 1985, Z PFLANZENK PFLANZEN, V92, P147
[10]   ORGANICS IN WATER - SOLID-PHASE EXTRACTION ON A SMALL-SCALE [J].
JUNK, GA ;
RICHARD, JJ .
ANALYTICAL CHEMISTRY, 1988, 60 (05) :451-454