SEROLOGIC DISCRIMINATION OF HUMAN T-CELL LYMPHOTROPIC VIRUS-INFECTION BY USING A SYNTHETIC PEPTIDE-BASED ENZYME-IMMUNOASSAY

被引：56

作者：

LAL, RB ^{[1
]}

RUDOLPH, DL ^{[1
]}

LAIRMORE, MD ^{[1
]}

KHABBAZ, RF ^{[1
]}

GARFIELD, M ^{[1
]}

COLIGAN, JE ^{[1
]}

FOLKS, TM ^{[1
]}

机构：

[1] NIAID,BIOL RESOURCES BRANCH,BETHESDA,MD 20892

来源：

JOURNAL OF INFECTIOUS DISEASES | 1991年 / 163卷 / 01期

关键词：

D O I：

10.1093/infdis/163.1.41

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Synthetic peptides corresponding with unique regions of the envelope glycoproteins (gp46) of human T cell lymphotropic viruses (HTLVs) were used in an enzyme immunoassay to determine if HTLV-I and -II infections could be discriminated. Two synthetic HTLV-I sequence-derived peptides, Env-1 (amino acids 191-215) and Env-5 (amino acids 242-257), reacted with 92% and 100% of the serum specimens (n = 52) from HTLV-I-infected persons, respectively. Although a small percentage (8.6%) of serum specimens from persons infected with HTLV-II cross-reacted with Env-1, none of these specimens reacted with Env-5. Peptide Env-2 encoded by the envelope region of HTLV-II (amino acids 187-210) reacted with serum speciments from both HTLV-I (94%)- and HTLV-II (74%)-infected patients, whereas Env-6, another HTLV-II peptide (amino acids 238-254), reacted with < 6% of the specimens. Therefore, the Env-5 peptide with amino acid sequence SerProAsnValSerValProSerSerSerSerThuProLeuLeuTyr represents an immunodominant domain of HTLV-I that is recognized by serum antibodies from all HTLV-I-infected persons. Moreover, the Env-5-based ELISA allows a categorical distinction between the closely related HTLV-I and -II infections.

引用

页码：41 / 46

页数：6

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