SYNTHESIS OF 6-PHOSPHOGLUCONATE BY A COMBINATION OF HYDROGENASE AND 6-PHOSPHOGLUCONATE DEHYDROGENASE

被引:1
|
作者
HASUMI, F [1 ]
NAKADA, N [1 ]
OKURA, I [1 ]
机构
[1] TOKYO INST TECHNOL,DEPT BIOENGN,MEGURO KU,TOKYO 152,JAPAN
关键词
D O I
10.1246/nikkashi.1990.334
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The regeneration of nicotinamide-adenine dinucleotide (reduced form NADH) by the reduction of NAD+ with hydrogen gas was carried out in the presence of the hydrogenase from Alcaligenes eutrophus, and the synthesis of 6-phosphogluconate (6-PG) was attempted by a combination of the above system and 6-phosphogluconate dehydrogenase. After 24 h, the conversion of 2-fibulose 5-phosphate to 6-PG was 57.1% and turnover number of NAD+ was 2.8. © 1990, The Chemical Society of Japan. All rights reserved.
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页码:334 / 336
页数:3
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