BETA-ADRENERGIC RECEPTOR-MEDIATED CALCIUM MOBILIZATION IN THE HUMAN JURKAT T-CELL LINE

被引：10

作者：

TAKEMURA, H ^{[1
]}

HATTA, S ^{[1
]}

YAMADA, K ^{[1
]}

OHSHIKA, H ^{[1
]}

机构：

[1] SAPPORO MED UNIV,SCH HLTH SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

LIFE SCIENCES | 1995年 / 56卷 / 18期

关键词：

JURKAT CELL; BETA-ADRENERGIC RECEPTOR; CAMP; CALCIUM MOBILIZATION;

D O I：

10.1016/0024-3205(95)00107-H

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The regulation of Ca2+ mobilization by beta-adrenergic receptor in the human Jurkat T cell line was investigated. Jurkat cells had a single class of beta-adrenergic receptor binding sites. Isoproterenol (ISP) caused the increase in cytosolic free Ca2+ concentration ([Ca2+](i)) in a dose-dependent manner. In the absence of extracellular Ca2+, the pretreatment with OKT3, an anti-CD3 antibody, did not affect a transient increase in [Ca2+](i) induced by the subsequent addition of ISP, and vice versa. On the other hand, the pretreatment with thapsigargin abolished the response of [Ca2+](i) to the subsequent additions of ISP and OKT3. In permeabilized Jurkat cells, the addition of cAMP released Ca2+ from the intracellular Ca2+ pool. Neither nimodipine nor H8, a protein kinase A inhibitor, affected the increase in [Ca2+](i) induced by ISP. The results suggest that cAMP accumulated by the activation of beta-adrenergic receptor may directly release Ca2+ from the inositol trisphosphate-insensitive intracellular Ca2+ pool in Jurkat T cells.

引用

页码：1443 / 1454

页数：12