BETA-ADRENERGIC RECEPTOR-MEDIATED CALCIUM MOBILIZATION IN THE HUMAN JURKAT T-CELL LINE

被引：10

作者：

TAKEMURA, H ^{[1
]}

HATTA, S ^{[1
]}

YAMADA, K ^{[1
]}

OHSHIKA, H ^{[1
]}

机构：

[1] SAPPORO MED UNIV,SCH HLTH SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

LIFE SCIENCES | 1995年 / 56卷 / 18期

关键词：

JURKAT CELL; BETA-ADRENERGIC RECEPTOR; CAMP; CALCIUM MOBILIZATION;

D O I：

10.1016/0024-3205(95)00107-H

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The regulation of Ca2+ mobilization by beta-adrenergic receptor in the human Jurkat T cell line was investigated. Jurkat cells had a single class of beta-adrenergic receptor binding sites. Isoproterenol (ISP) caused the increase in cytosolic free Ca2+ concentration ([Ca2+](i)) in a dose-dependent manner. In the absence of extracellular Ca2+, the pretreatment with OKT3, an anti-CD3 antibody, did not affect a transient increase in [Ca2+](i) induced by the subsequent addition of ISP, and vice versa. On the other hand, the pretreatment with thapsigargin abolished the response of [Ca2+](i) to the subsequent additions of ISP and OKT3. In permeabilized Jurkat cells, the addition of cAMP released Ca2+ from the intracellular Ca2+ pool. Neither nimodipine nor H8, a protein kinase A inhibitor, affected the increase in [Ca2+](i) induced by ISP. The results suggest that cAMP accumulated by the activation of beta-adrenergic receptor may directly release Ca2+ from the inositol trisphosphate-insensitive intracellular Ca2+ pool in Jurkat T cells.

引用

页码：1443 / 1454

页数：12

共 35 条

[31] Beta 2 adrenergic receptor and mu opioid receptor interact to potentiate the aggressiveness of human breast cancer cell by activating the glycogen synthase kinase 3 signaling [J].

Rousseau, Benedicte ;

Murugan, Sengottuvelan ;

Palagani, Ajay ;

Sarkar, Dipak K. .

BREAST CANCER RESEARCH, 2022, 24 (01)

[32] Regulation of T Helper Cell Type 2 Immune Response by Controlling Beta-2 Adrenergic Receptor in Dendritic Cells of Patients with Allergic Rhinitis [J].

Yeon, Ji Woo ;

Kim, Byoungjae ;

Byun, Junhyoung ;

Jung, Semyoung ;

Park, Jaehyung ;

Han, Munsoo ;

Baek, Seung-Kuk ;

Kim, Tae Hoon .

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2023, 184 (12) :1173-1183

[33] Andrographolide inhibits growth of human T-cell acute lymphoblastic leukemia Jurkat cells by downregulation of PI3K/AKT and upregulation of p38 MAPK pathways [J].

Yang, Tingfang ;

Yao, Shuluan ;

Zhang, Xianfeng ;

Guo, Yan .

DRUG DESIGN DEVELOPMENT AND THERAPY, 2016, 10 :1389-1397

[34] Inhibition of B cell receptor-mediated activation of primary human B cells by coengagement of CD19 and FcγRIIb with Fc-engineered antibodies [J].

Chu, Seung Y. ;

Vostiar, Igor ;

Karki, Sher ;

Moore, Gregory L. ;

Lazar, Greg A. ;

Pong, Erik ;

Joyce, Patrick F. ;

Szymkowski, David E. ;

Desjarlais, John R. .

MOLECULAR IMMUNOLOGY, 2008, 45 (15) :3926-3933

[35] Pharmacology of cloned human 5-HT1D receptor-mediated functional responses in stably transfected rat C6-glial cell lines: Further evidence differentiating human 5-HT1D and 5-HT1B receptors [J].

Pauwels, PJ ;

Palmier, C ;

Wurch, T ;

Colpaert, FC .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1996, 353 (02) :144-156

← 1 2 3 4 →