A MODEL OF CYTOSOLIC CALCIUM REGULATION AND AUTACOIDS PRODUCTION IN VASCULAR ENDOTHELIAL-CELL

被引：9
作者：
WONG, AYK [1 ]
KLASSEN, GA [1 ]
机构：
[1] DALHOUSIE UNIV,DEPT MED,HALIFAX B3H 4H2,NS,CANADA
来源：
BASIC RESEARCH IN CARDIOLOGY | 1992年 / 87卷 / 04期
关键词：
ENDOTHELIUM; CYTOSOLIC CALCIUM; AGONIST; EDRF; PROSTACYCLIN;
D O I：
10.1007/BF00796518
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A model of vascular endothelial cell is proposed to describe the mechanisms by which cytosolic calcium (Ca(i)) is modulated and endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2) are released when the cell is stimulated by agonist. The intracellular Ca2+ store of the model cell is comprised of a superficial (sc) and a deep (dc) compartment. The dc Ca2+ content is refilled by the sc whose [Ca2+] is the same as extracellular Ca2+. Inositol (1,4,5)-trisphosphate (IP3) produced by agonist modifies the dc permeability which discharges its Ca2+ to the cytosol. The increase of Ca(i) induces Ca2+ released from the sc. Ca2+-activated K+ current hyperpolarises the cell. The raised Ca(i) releases PGI2 in the presence of IP3 while EDRF is released by Ca(i). The model explains satisfactorily the Ca2+ transient and autacoids production of the aortic endothelial cell without the need of calcium influx from extracellular space. The cytoplasmic Ca2+ oscillations observed in human endothelial cell from umbilical veins were reproduced by the model. Production of EDRF by the artery due to increase in pressure was also simulated.
引用
收藏
页码：317 / 332
页数：16
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