PSEUDOMONAS-AERUGINOSA ANTIBODIES IN HUMAN-PLASMA

被引：0

作者：

DOI, T ^{[1
]}

YOSHIOKA, M ^{[1
]}

NAKAJIMA, T ^{[1
]}

机构：

[1] GREEN CROSS CORP, CENT RES LABS, MIYAKOJIMA KU, OSAKA 534, JAPAN

来源：

JAPANESE JOURNAL OF EXPERIMENTAL MEDICINE | 1976年 / 46卷 / 03期

关键词：

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Plasma samples from healthy adults were examined for antibodies to the common protective antigen (OEP [original endotoxin protein]) by the passive hemagglutination (HA) reaction. The majority of them had an antibody titer of 60 or lower. 2-Mercaptoethanol (2-ME) treatment caused a decrease in HA titer down to 16 or lower in most of the cases, suggesting that the OEP antibody resides mostly in IgM [immunoglobulin M] and slightly in IgG. This finding was corroborated by gel filtration on Sephadex G-200. IgA is probably not implicated in the HA titer since IgA was HA negative. However, the detection of enzymatic activity by the enzyme-linked immunosorbent assay (ELISA) suggests a possibility that OEP antibody resides in IgA. Antibodies to proteolytic enzymes, especially P. aeruginosa protease and elastase, were present, in most cases, at a titer of 16 or lower, as determined by the HA reaction. These antibodies are mainly IgM.

引用

页码：149 / 154

页数：6