CDNA CLONING AND EXPRESSION OF A POTATO (SOLANUM-TUBEROSUM) INVERTASE

被引：40

作者：

HEDLEY, PE ^{[1
]}

MACHRAY, GC ^{[1
]}

DAVIES, HV ^{[1
]}

BURCH, L ^{[1
]}

WAUGH, R ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

PLANT MOLECULAR BIOLOGY | 1993年 / 22卷 / 05期

关键词：

BETA-FRUCTOSIDASE; BETA-FRUCTOFURANOSIDASE; RT-PCR;

D O I：

10.1007/BF00027378

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots.

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页码：917 / 922

页数：6

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