ROLE OF A RAS HOMOLOG IN THE LIFE-CYCLE OF SCHIZOSACCHAROMYCES POMBE

被引：234
作者：
FUKUI, Y
KOZASA, T
KAZIRO, Y
TAKEDA, T
YAMAMOTO, M
机构：
[1] UNIV TOKYO, INST MED SCI, DEPT CHEM, PO TAKANAWA, TOKYO 108, JAPAN
[2] UNIV TOKYO, INST MED SCI, MOLEC GENET LAB, TOKYO 108, JAPAN
来源：
CELL | 1986年 / 44卷 / 02期
关键词：
D O I：
10.1016/0092-8674(86)90767-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have analyzed the function of the only ras homolog in S. pombe detectable by Southern blotting, ras1, which is homologous to mammalian ras genes and has been cloned. We have disrupted the ras1 gene and have replaced it with ras1Val17, which corresponds to a transforming variant of mammalian ras. Loss of ras1 activity by disruption results in the complete inability to mate. The cell body of a ras1- strain is extensively deformed, and a ras1-/ras1- diploid sporulates very poorly. Unlike RAS1 and RAS2 of S. cerevisiae, ras1 of S. pombe appears to have no effect on adenylate cyclase activity. This suggests that the target enzymes presumably modulated by ras proteins in signal transduction are not the same for all organisms.
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页码：329 / 336
页数：8
相关论文
共 57 条
[1]   HIGH-FREQUENCY TRANSFORMATION OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].
BEACH, D ;
NURSE, P .
NATURE, 1981, 290 (5802) :140-142
[2]   DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].
BEAUCAGE, SL ;
CARUTHERS, MH .
TETRAHEDRON LETTERS, 1981, 22 (20) :1859-1862
[3]   STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].
BOTSTEIN, D ;
FALCO, SC ;
STEWART, SE ;
BRENNAN, M ;
SCHERER, S ;
STINCHCOMB, DT ;
STRUHL, K ;
DAVIS, RW .
GENE, 1979, 8 (01) :17-24
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   ACTIVATION OF KI-RAS 2 GENE IN HUMAN-COLON AND LUNG CARCINOMAS BY 2 DIFFERENT POINT MUTATIONS [J].
CAPON, DJ ;
SEEBURG, PH ;
MCGRATH, JP ;
HAYFLICK, JS ;
EDMAN, U ;
LEVINSON, AD ;
GOEDDEL, DV .
NATURE, 1983, 304 (5926) :507-513
[6]   COMPLETE NUCLEOTIDE-SEQUENCES OF THE T24 HUMAN BLADDER-CARCINOMA ONCOGENE AND ITS NORMAL HOMOLOG [J].
CAPON, DJ ;
CHEN, EY ;
LEVINSON, AD ;
SEEBURG, PH ;
GOEDDEL, DV .
NATURE, 1983, 302 (5903) :33-37
[7]  
Cryer D R, 1975, Methods Cell Biol, V12, P39
[8]   RAS-RELATED GENE-SEQUENCES IDENTIFIED AND ISOLATED FROM SACCHAROMYCES-CEREVISIAE [J].
DEFEOJONES, D ;
SCOLNICK, EM ;
KOLLER, R ;
DHAR, R .
NATURE, 1983, 306 (5944) :707-709
[9]   MAMMALIAN AND YEAST RAS GENE-PRODUCTS - BIOLOGICAL FUNCTION IN THEIR HETEROLOGOUS SYSTEMS [J].
DEFEOJONES, D ;
TATCHELL, K ;
ROBINSON, LC ;
SIGAL, IS ;
VASS, WC ;
LOWY, DR ;
SCOLNICK, EM .
SCIENCE, 1985, 228 (4696) :179-184
[10]   A MEMBRANE-FILTER TECHNIQUE FOR DETECTION OF COMPLEMENTARY DNA [J].
DENHARDT, DT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 23 (05) :641-&
123456