INTERACTION OF BUTYLATED HYDROXYANISOLE WITH MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION

被引:35
作者
FUSI, F
SGARAGLI, G
MURPHY, MP
机构
[1] UNIV DUBLIN TRINITY COLL,DEPT BIOCHEM,DUBLIN 2,IRELAND
[2] UNIV SIENA,INST SCI FARMACOL,I-53100 SIENNA,ITALY
关键词
D O I
10.1016/0006-2952(92)90493-3
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The antioxidant, butylated hydroxyanisole (BHA), has a number of effects on mitochondrial oxidative phosphorylation. In this study we apply the novel approach developed by Brand (Brand MD, Biochim Biophys Acta 1018: 128-133, 1990) to investigate the site of action of BHA on oxidative phosphorylation in rat liver mitochondria. Using this approach we show that BHA increases the proton leak through the mitochondrial inner membrane and that it also inhibits the DELTA-p (proton motive force across the mitochondrial inner membrane) generating system, but has no effect on the phosphorylation system. This demonstrates that compounds having pleiotypic effects on mitochondrial oxidative phosphorylation in vitro can be analysed and their many effects distinguished. This approach is of general use in analysing many other compounds of pharmacological interest which interact with mitochondria. The implications of these results for the mechanism of interaction of BHA with mitochondrial oxidative phosphorylation are discussed.
引用
收藏
页码:1203 / 1208
页数:6
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