COCLUSTERING CD45 WITH CD4 OR CD8 ALTERS THE PHOSPHORYLATION AND KINASE-ACTIVITY OF P56LCK

被引:165
作者
OSTERGAARD, HL [1 ]
TROWBRIDGE, IS [1 ]
机构
[1] SALK INST BIOL STUDIES,DEPT CANC BIOL,POB 85800,SAN DIEGO,CA 92138
关键词
D O I
10.1084/jem.172.1.347
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Antibody-mediated CD4 crosslinking results in increased tyrosine phosphorylation and tyrosine kinase activity of the associated p56kk. Treatment with anti-CD4 and anti-Ig also induced the phosphorylation of p56kk in a CD45− mutant cell line, indicating that the increase in phosphotyrosine content of p56kk is not the result of being sequestered from CD45 protein tyrosine phosphatase (PTPase). Antibody-mediated coclustering of CD45 with CD4 inhibited the anti-CD4-induced phosphorylation of p56kk on tyrosine and the concomitant increase in in vitro kinase activity. Similar results were obtained when CD45 was coclustered with CD8 on cytotoxic T cell lines. These observations provide strong evidence that p56kk is a substrate for CD45 in vivo and provide an assay to study the regulation and specificity ofCD45 PTPase activity. © 1990, Rockefeller University Press., All rights reserved.
引用
收藏
页码:347 / 350
页数:4
相关论文
共 14 条
[1]   THE LEUKOCYTE COMMON ANTIGEN (CD45) - A PUTATIVE RECEPTOR-LINKED PROTEIN TYROSINE PHOSPHATASE [J].
CHARBONNEAU, H ;
TONKS, NK ;
WALSH, KA ;
FISCHER, EH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7182-7186
[2]   MURINE LYMPHOCYTE-T CLONES WITH DISTINCT IMMUNOLOGICAL FUNCTIONS [J].
GLASEBROOK, AL ;
SARMIENTO, M ;
LOKEN, MR ;
DIALYNAS, DP ;
QUINTANS, J ;
EISENBERG, L ;
LUTZ, CT ;
WILDE, D ;
FITCH, FW .
IMMUNOLOGICAL REVIEWS, 1981, 54 :225-266
[3]  
HURLEY TR, 1989, ONCOGENE, V4, P265
[4]   ANTIGEN-INDUCIBLE, H-2-RESTRICTED, INTERLEUKIN-2-PRODUCING T-CELL HYBRIDOMAS - LACK OF INDEPENDENT ANTIGEN AND H-2 RECOGNITION [J].
KAPPLER, JW ;
SKIDMORE, B ;
WHITE, J ;
MARRACK, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 153 (05) :1198-1214
[5]   EXPRESSION OF CD45 ALTERS PHOSPHORYLATION OF THE LCK-ENCODED TYROSINE PROTEIN-KINASE IN MURINE LYMPHOMA T-CELL LINES [J].
OSTERGAARD, HL ;
SHACKELFORD, DA ;
HURLEY, TR ;
JOHNSON, P ;
HYMAN, R ;
SEFTON, BM ;
TROWBRIDGE, IS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8959-8963
[6]  
OVAL J, 1989, LEUK RES S1, V13, P13
[7]   THE CD4 RECEPTOR IS COMPLEXED IN DETERGENT LYSATES TO A PROTEIN-TYROSINE KINASE (PP58) FROM HUMAN LYMPHOCYTES-T [J].
RUDD, CE ;
TREVILLYAN, JM ;
DASGUPTA, JD ;
WONG, LL ;
SCHLOSSMAN, SF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5190-5194
[8]   MOLECULAR-INTERACTIONS, T-CELL SUBSETS AND A ROLE OF THE CD4/CD8-P561CK COMPLEX IN HUMAN T-CELL ACTIVATION [J].
RUDD, CE ;
ANDERSON, P ;
MORIMOTO, C ;
STREULI, M ;
SCHLOSSMAN, SF .
IMMUNOLOGICAL REVIEWS, 1989, 111 :225-266
[9]  
THOMAS ML, 1989, ANNU REV IMMUNOL, V7, P339, DOI 10.1146/annurev.immunol.7.1.339
[10]  
TONKS NK, 1988, BIOCHEMISTRY-US, V27, P8696