ISOLATION OF THERMOTOLERANT, FERMENTATIVE YEASTS GROWING AT 52-DEGREES-C AND PRODUCING ETHANOL AT 45-DEGREES-C AND 50-DEGREES-C

被引:175
作者
BANAT, IM
NIGAM, P
MARCHANT, R
机构
[1] Department of Biology, University of Al-Ain, Abo-Dhabi
[2] Department of Biological and Biomedical Sciences, University of Ulster, Coleraine
关键词
ETHANOL PRODUCTION; FERMENTATIVE YEAST; KLUYVEROMYCES; THERMOTOLERANT YEAST;
D O I
10.1007/BF01201874
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Five thermotolerant, alcohol-producing yeast cultures were isolated from samples obtained from India. Two were identified as of Kluyveromyces marxianus. All five grew on plate-cultures up to 52-degrees-C, with maximum growth rates in liquid culture at 40-degrees-C. All produced relatively high alcohol concentrations: 5.7 to 7.0% (w/v) at 45-degrees-C and 5.0 to 5.5% (w/v) at 50-degrees-C when growing on 14.0% (w/v) glucose. All five isolates fermented diluted molasses containing 16.0% (w/v) total sugars, producing 5.6 to 6.0% (w/v) alcohol concentrations. Supplementing the molasses with P, K, Mg and Mn resulted in a 13 to 20% increase in alcohol production at 40-degrees-C. The maximum amounts of alcohol produced on supplemented molasses were 7.5 to 8.0 and 6.5 to 7.0% (w/v) at 37-degrees-C and 40-degrees-C, respectively.
引用
收藏
页码:259 / 263
页数:5
相关论文
共 19 条
  • [11] Hughes D.B., Tudroszen N.J., Moye C.J., The effect of temperature on the kinetics of ethanol production by a thermotolerant strain of Kluveromyces marxianus, Biotechnology Letters, 6, pp. 1-6, (1984)
  • [12] Krouwel P.G., Braber L., Ethanol production by yeast at supraoptimal temperatures, Biotechnology Letters, 1, pp. 403-408, (1979)
  • [13] Raabo E., Terkildsen T.C., On the enzymic determination of blood glucose, Scandinavian Journal for Clinical Laboratory Investigation, 12, pp. 402-405, (1960)
  • [14] Rose D., Yeasts for molasses alcohol, Process Biochemistry, 3, pp. 10-12, (1976)
  • [15] Ryu B.H., Nam K.D., Kim H.S., Kim D.S., Ji Y.A., Jung S.T., Screening of thermotolerant yeast strain for ethanol fermentation, Korean Journal of Applied Microbiology and Bioengineering, 16, pp. 265-269, (1988)
  • [16] Seki T., Myoga S., Limtong S., Vedon S., Kumnuanta J., Taguchi H., Genetic construction of yeast strains for high ethanol production, Biotechnology Letters, 5, pp. 351-356, (1983)
  • [17] Spindler D.D., Wyman C.E., Grohmann K., Evaluation of thermotolerant yeasts in controlled simultaneous saccharification and fermentation of cellulose to ethanol, Biochnology and Bioengineering, 34, pp. 189-195, (1988)
  • [18] Suutari K.L., Laakso S., Temperature adaptation in yeast: the role of fatty acids, Journal of General Microbiology, 136, pp. 1469-1474, (1990)
  • [19] Szczodrak J., Targonski Z., Selection of thermotolerant yeast strains for simultaneous saccharification and fermentation of cellulose, Biotechnology and Bioengineering, 31, pp. 300-303, (1988)