A DNA PROBE WHICH DISTINGUISHES GLOBODERA-PALLIDA PA2/3 FROM G-ROSTOCHIENSIS RO1 AND OTHER CYST-FORMING NEMATODES

被引：2

作者：

MARSHALL, JW

CRAWFORD, AM

机构：

[1] Crop and Food Research Private Bag, Christchurch

[2] AgResearch Molecular Biology Unit, Biochemistry Department, University of Otago, Dunedin

来源：

NEW ZEALAND JOURNAL OF ZOOLOGY | 1992年 / 19卷 / 3-4期

关键词：

DNA PROBES; GLOBODERA-PALLIDA; G-ROSTOCHIENSIS; SPECIFICITY; SENSITIVITY;

D O I：

10.1080/03014223.1992.10422317

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A small DNA fragment (approx. 350 base pairs) from the genome of the potato cyst nematode Globodera pallida Pa2/3 was cloned in a bacterial plasmid. When used as a probe in dot-blot DNA hybridisations against a range of nematodes, the cloned DNA bound to G. pallida Pa2/3 but not to Globodera rostochiensis Ro1. The cereal cyst nematode Heterodera avenae, the clover cyst nematode Heterodera trifolii, the root knot nematodes Meloidogyne hapla and Meloidogyne incognita, and the beet cyst nematode Heterodera schactii did not cross-hybridise. This probe can detect as few as six larvae of G. pallida.

引用

页码：133 / 138

页数：6