INVITRO TOXICITY TESTING FOR ANTIBACTERIALS AGAINST HUMAN KERATINOCYTES

被引：34

作者：

SMOOT, EC ^{[1
]}

KUCAN, JO ^{[1
]}

ROTH, A ^{[1
]}

MODY, N ^{[1
]}

DEBS, N ^{[1
]}

机构：

[1] MEM MED CTR, DEPT PATHOL, SPRINGFIELD, IL 62781 USA

来源：

PLASTIC AND RECONSTRUCTIVE SURGERY | 1991年 / 87卷 / 05期

关键词：

D O I：

10.1097/00006534-199105000-00017

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The use of cultured human keratinocytes in an in vitro comparison of topical antibacterial toxicity for epithelial cells was examined. The complement of three assessments allows testing of epithelial migration, growth, and survival. The three assessments include (1) flow cytometry for determination of cell survival, (2) a comparison of and (3) an evaluation of cell migration using a technique of dermal explants to study radial migration. A comparative ranking of the toxicities of the various topical antibacterials was determined with the three assessments. This has confirmed anecdotal reports that many of the topical antibacterials are cell-toxic and may inhibit wound healing. This information can be directly extrapolated to the clinical setting, unlike many of the animal data for wound healing that currently exist.

引用

页码：917 / 924

页数：8

共 16 条

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