IMMUNOAFFINITY COLUMN CLEANUP FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AFLATOXINS B-1, B-2, G(1), G(2), M(1) AND Q(1) IN URINE

被引：40

作者：

KUSSAK, A ^{[1
]}

ANDERSSON, B ^{[1
]}

ANDERSSON, K ^{[1
]}

机构：

[1] NATL INST OCCUPAT HLTH,DIV ANALYT CHEM,S-90713 UMEA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00227-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of aflatoxins B-1, B-2, G(1), G(2), M(1) and Q(1) in human urine has been developed. The 10-ml urine samples were automatically cleaned up on immunoaffinity columns and analysed by highperformance liquid chromatography (HPLC), including post-column derivatization with bromine and fluorescence detection. Average affatoxin recoveries were: B-1 103%, B-2 106%, G(1) 98% and G(2) 96% in the range 6.8-73 pg/ml of urine and M(1) 103% and Q(1) 100% in the range 18-97 pg/ml of urine. The relative standard deviations were all between 1% and 21%. The determination limits of aflatoxins in urine were 6.8 pg/ml for B-1, B-2, G(1) and G(2) and 18 pg/ml for M(1) and Q(1).

引用

页码：253 / 259

页数：7

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