TYPING OF THE GRANULOCYTE-SPECIFIC NA ANTIGENS BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS

被引：12

作者：

STEIN, EL ^{[1
]}

BUX, J ^{[1
]}

SANTOSO, S ^{[1
]}

MUELLERECKHARDT, C ^{[1
]}

机构：

[1] UNIV GIESSEN,INST CLIN IMMUNOL & TRANSFUS MED,D-35392 GIESSEN,GERMANY

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1994年 / 87卷 / 02期

关键词：

NA ANTIGENS; PCR-RFLP; ALLOANTIGENS; GRANULOCYTES; NEUTROPHILS;

D O I：

10.1111/j.1365-2141.1994.tb04939.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An RNA-based method has been developed to genotype donors for the granulocyte-specific alloantigens NA1 and NA2. mRNA was isolated from granulocytes, reversely transcribed into cDNA and amplified using an Fc-gamma-receptor III-1 sequence-specific primer in the polymerase chain reaction (PCR). PCR products were analysed by restriction fragment length polymorphism (RFLP) using the restriction endonuclease Tag I, which provided a distinct restriction fragment pattern corresponding to the NA alleles. 17 donors were typed by PCR-RFLP and the results were in close accordance with those obtained by serological phenotyping by granulocyte immunofluorescence and the antigen capture assay MAIGA.

引用

页码：428 / 430

页数：3

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