IMAGING FLAGELLA OF HALOBACTERIA BY ATOMIC-FORCE MICROSCOPY

被引：8
|
作者：
JASCHKE, M [1 ]
BUTT, HJ [1 ]
WOLFF, EK [1 ]
机构：
[1] INST TECHNOLOGIEENTWICKLUNG & SYST ANAL,D-58453 WITTEN,GERMANY
来源：
ANALYST | 1994年 / 119卷 / 09期
关键词：
HALOBACTERIA; FLAGELLA; ATOMIC FORCE MICROSCOPY; FLAGELLAR MOTOR SUPER-FLAGELLA; TIP BROADENING;
D O I：
10.1039/an9941901943
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Halobacterial flagella were adsorbed on mica and imaged with an atomic force microscope in air. Different assemblies such as large, net-like structures, and straight flagella aligned by flow were observed. The hypothesis of individual flagellar motors was confirmed by frequent observation of knobs at the end of single flagella. A periodic substructure was observed along flagellar strands with a spacing of 50-100 nm. The diameter of halobacterial flagella was determined as 11 +/- 4 nm. The influence of two different preparations on the flagellar profile, and reasons for the distortion of height and width are discussed.
引用
收藏
页码：1943 / 1946
页数：4
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