IMAGING FLAGELLA OF HALOBACTERIA BY ATOMIC-FORCE MICROSCOPY

被引：9

作者：

JASCHKE, M ^{[1
]}

BUTT, HJ ^{[1
]}

WOLFF, EK ^{[1
]}

机构：

[1] INST TECHNOLOGIEENTWICKLUNG & SYST ANAL,D-58453 WITTEN,GERMANY

来源：

ANALYST | 1994年 / 119卷 / 09期

关键词：

HALOBACTERIA; FLAGELLA; ATOMIC FORCE MICROSCOPY; FLAGELLAR MOTOR SUPER-FLAGELLA; TIP BROADENING;

D O I：

10.1039/an9941901943

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Halobacterial flagella were adsorbed on mica and imaged with an atomic force microscope in air. Different assemblies such as large, net-like structures, and straight flagella aligned by flow were observed. The hypothesis of individual flagellar motors was confirmed by frequent observation of knobs at the end of single flagella. A periodic substructure was observed along flagellar strands with a spacing of 50-100 nm. The diameter of halobacterial flagella was determined as 11 +/- 4 nm. The influence of two different preparations on the flagellar profile, and reasons for the distortion of height and width are discussed.

引用

页码：1943 / 1946

页数：4

共 23 条

[1] MORPHOLOGY, FUNCTION AND ISOLATION OF HALOBACTERIAL FLAGELLA [J].

ALAM, M ;

OESTERHELT, D .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 176 (04) :459-475

[2] PURIFICATION, RECONSTITUTION AND POLYMORPHIC TRANSITION OF HALOBACTERIAL FLAGELLA [J].

ALAM, M ;

OESTERHELT, D .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (03) :495-499

[3] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[4] IMAGING CELLS WITH THE ATOMIC FORCE MICROSCOPE [J].

BUTT, HJ ;

WOLFF, EK ;

GOULD, SAC ;

NORTHERN, BD ;

PETERSON, CM ;

HANSMA, PK .

JOURNAL OF STRUCTURAL BIOLOGY, 1990, 105 (1-3) :54-61

[5] PAUSING, SWITCHING AND SPEED FLUCTUATION OF THE BACTERIAL FLAGELLAR MOTOR AND THEIR RELATION TO MOTILITY AND CHEMOTAXIS [J].

EISENBACH, M ;

WOLF, A ;

WELCH, M ;

CAPLAN, SR ;

LAPIDUS, IR ;

MACNAB, RM ;

ALONI, H ;

ASHER, O .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 211 (03) :551-563

[6] BIOLOGICAL APPLICATIONS OF SCANNING PROBE MICROSCOPES [J].

ENGEL, A .

ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1991, 20 :79-108

[7]

HOH J H, 1992, Trends in Cell Biology, V2, P208, DOI 10.1016/0962-8924(92)90248-L

[8] FLAGELLA, GAS VACUOLES AND CELL-WALL STRUCTURE IN HALOBACTERIUM-HALOBIUM - AN ELECTRON MICROSCOPE STUDY [J].

HOUWINK, AL .

JOURNAL OF GENERAL MICROBIOLOGY, 1956, 15 (01) :146-150

[9]

KELLENBERGER E, 1987, CRYOTECHNIQUES BIOL, P50

[10] BACTERIAL FLAGELLA ROTATING IN BUNDLES - STUDY IN HELICAL GEOMETRY [J].

MACNAB, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (01) :221-225

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