Virulence factors of Escherichia coli strains isolated from diarrheic children: Influence of storage conditions.

被引:0
作者
Giugliano, LG
Araujo, AN
Piva, IC
Inham, TM
Ferraz, LR
Guth, BC
机构
来源
REVISTA DE MICROBIOLOGIA | 1995年 / 26卷 / 04期
关键词
virulence factors; enterotoxins; cytotoxins; cytotoxic necrotizing factor (CNF);
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The frequency of isolation of enterotoxigenic (ETEC), verotoxigenic (VTEC) and necrotizing Escherichia coli (NTEC) was determined in 167 children with diarrhea from a central area of Brazil. E.coli strains producing verotoxin (VT), cytotoxic necrotizing factor (CNF) and thermolabile enterotoxin (LT) were found in 3%, 3.6% and 7.2% of the cases, respectively. The E.coli strains were also assayed for their ability to induce mannose resistant haemagglutination (MRHA) of human and bovine erythrocytes. MRHA+ strains were observed in 42% of the cases and colonization factor antigens I and II were identified in 32% of the MRHA+ cases. After long term storage at room temperature, the stability of virulence factors produced by 28 selected isolates was evaluated. Production of cytotoxins was maintained in 3 of the 5 VT+ and in 5 of the 6 CNF+ isolates. Expression of LT was maintained in 9 of the 12 LT+ isolates. Of the 23 CFA+ strains, only 7 maintained the haemagglutination pattern. Expression of ST was detected in 4 strains and, besides those, 4 further ST+ strains were recognized by colony hybridization. The LT genotype was found in 11 of the selected isolates. In conclusion, by studying strains of E. coli isolated from children's diarrhea, NTEC strains were identified for the first time in Brazil. This study also confirms the great importance of storage conditions for the detection of virulence factors of ETEC and shows that VTEC and NTEC may lose its cytotoxin producing ability after prolonged storage at room temperature.
引用
收藏
页码:267 / 273
页数:7
相关论文
共 50 条
[21]   DISTRIBUTION OF VIRULENCE FACTORS IN UROPATHOGENIC ESCHERICHIA COLI ISOLATED IN KERMANSHAH [J].
Mohajeri, Parviz ;
Khademi, Hosna ;
Farahani, Abbas ;
Ebrahimi, Roya .
IRANIAN JOURNAL OF PUBLIC HEALTH, 2014, 43 :106-106
[22]   EVALUATION OF VIRULENCE FACTORS AND THE ADHESIVE CAPABILITY OF ESCHERICHIA-COLI STRAINS [J].
SAVOIA, D ;
MILLESIMO, M ;
FONTANA, G .
MICROBIOS, 1994, 80 (323) :73-81
[23]   Serogroups and virulence genes of Escherichia coli isolated from psittacine birds [J].
Knoebl, Terezinha ;
Saidenberg, Andre B. S. ;
Moreno, Andrea M. ;
Gomes, Tania A. T. ;
Vieira, Monica A. M. ;
Leite, Domingos S. ;
Blanco, Jesus E. ;
Ferreira, Antonio J. P. .
PESQUISA VETERINARIA BRASILEIRA, 2011, 31 (10) :916-921
[24]   VIRULENCE FACTORS IN ESCHERICHIA-COLI ISOLATED FROM BLOOD AND CEREBROSPINAL-FLUID [J].
DIAS, AMG ;
KANO, E ;
NAKAHARA, LK ;
FERNANDES, SA ;
KATO, MAMF ;
IRINO, K .
REVISTA DE MICROBIOLOGIA, 1994, 25 (02) :77-82
[25]   Frequency of virulence factors in Escherichia coli isolated from suckling pigs with diarrhoea in China [J].
Liu, Wenxin ;
Yuan, Chaowen ;
Meng, Xiangqiu ;
Du, Yuance ;
Gao, Ruize ;
Tang, Jie ;
Shi, Dongfang .
VETERINARY JOURNAL, 2014, 199 (02) :286-289
[26]   Virulence factors of avian pathogenic Escherichia coli isolated from broilers from the south of Brazil [J].
da Rocha, ACGP ;
da Silva, AB ;
de Brito, BG ;
Moraes, HLD ;
Pontes, AP ;
Cé, MC ;
do Nascimento, VP ;
Salle, CTP .
AVIAN DISEASES, 2002, 46 (03) :749-753
[27]   Expression of virulence factors among Escherichia coli isolated from the periurethra and urine of children with neurogenic bladder on intermittent catheterization [J].
Schlager, TA ;
Whittam, TS ;
Hendley, JO ;
Wilson, RA ;
Bhang, J ;
Grady, R ;
Stapleton, A .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2000, 19 (01) :37-41
[28]   Investigation of Antibiotic Susceptibility and Virulence Genes in Escherichia coli Strains Isolated from Blood and Urine Samples [J].
Hasanli, Laman ;
Dagi, Hatice Turk ;
Arslan, Ugur .
JOURNAL OF PEDIATRIC INFECTIOUS DISEASES, 2022, 17 (02) :98-105
[29]   Distribution of virulence factors and its relatedness towards the antimicrobial response of enterotoxigenic Escherichia coli strains isolated from patients in Kolkata, India [J].
Mondal, Indranil ;
Bhakat, Debjyoti ;
Chowdhury, Goutam ;
Manna, Asis ;
Samanta, Sandip ;
Deb, Alok Kumar ;
Mukhopadhyay, Asish Kumar ;
Chatterjee, Nabendu Sekhar .
JOURNAL OF APPLIED MICROBIOLOGY, 2022, 132 (01) :675-686
[30]   Antibiotic resistance, virulence factors and biofilm formation ability in Escherichia coli strains isolated from chicken meat and wildlife in the Czech Republic [J].
Pavlickova, Silvie ;
Klancnik, Anja ;
Dolezalova, Magda ;
Mozina, Sonja Smole ;
Holko, Ivan .
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES, 2017, 52 (08) :570-576