HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PLASMA TRIGLYCERIDE TYPE COMPOSITION IN A NORMAL POPULATION OF BARCELONA - RELATIONSHIP WITH AGE, SEX AND OTHER PLASMA-LIPID PARAMETERS

A coupled TLC-HPLC procedure is proposed for the separation and determination of plasma triglycerides. The method was tested by application to plasma samples corresponding to a normal population of Barcelona (Spain). Eighteen different triglyceride types were identified and their relative proportions were established, in order to give a ''normal profile'' for men and women. Sex-related differences (p < 0.05) were only found for dioleostearin and palmitodilinolein + linoleooleopalmitolein (LLP + LOPa). A correlation study showed that palmitodiolein and total cholesterol levels increase with age, whereas LLP-LOPa decreases in men and palmitolinoleoolein + palmitooleopalmitolein in women.