CLONING AND EXPRESSION OF THE PLASMID-ENCODED BENZENE DIOXYGENASE GENES FROM PSEUDOMONAS-PUTIDA ML2

被引：24

作者：

TAN, HM ^{[1
]}

MASON, JR ^{[1
]}

机构：

[1] KINGS COLL,DIV BIOSPHERE SCI,MICROBIAL PHYSIOL RES GRP,CAMPDEN HILL RD,LONDON W8 7AH,ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 72卷 / 03期

关键词：

bedABC1C2; Benzene dioxygenase; Catabolic plasmid;

D O I：

10.1016/0378-1097(90)90314-G

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hybridization using heterologous dioxygenase gene probes indicated the presence of the genes encoding the enzyme benzene dioxygenase on a 112 kb plasmid from Pseudomonas putida ML2. They were identified as benzene dioxygenase genes (bedABC1C2) by cloning in Escherichia coli and analysis of expression by Western blotting using antibodies raised to the four polypeptides of purified benzene dioxygenase. © 1990.

引用

页码：259 / 264

页数：6

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