SURVIVAL OF INTRACEREBRALLY GRAFTED RAT DOPAMINE NEURONS PREVIOUSLY CULTURED INVITRO

被引：46

作者：

BRUNDIN, P ^{[1
]}

BARBIN, G ^{[1
]}

ISACSON, O ^{[1
]}

MALLAT, M ^{[1
]}

CHAMAK, B ^{[1
]}

PROCHIANTZ, A ^{[1
]}

GAGE, FH ^{[1
]}

BJORKLUND, A ^{[1
]}

机构：

[1] COLL FRANCE, INSERM, U114, GRP NB, F-75231 PARIS 05, FRANCE

来源：

NEUROSCIENCE LETTERS | 1985年 / 61卷 / 1-2期

关键词：

D O I：

10.1016/0304-3940(85)90404-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Fetal rat dopamine (DA) neurons were cultured in vitro for a 6-day period and transported, after redisssociation, for 2 days prior to being grafted to the neostriatum of adult rats with 6-hydroxydopamine lesions of the ascending nigrostriatal pathway. In 2 of the 5 graft recipients that were tested for amphetamine-induced motor asymmetry, the grafts eliminated the lesion-induced turning behaviour within 3-6 weeks after transplantation. Fluorescence histochemistry revealed surviving grafts in all 6 recipients at 7 weeks after transplantation, containing between 42 and 125 DA neurons. The number of surviving DA neurons in the 3 non-compensated rats was below the minimum number of cells previously found to be necessary for functional effects on turning behaviour to occur.

引用

页码：79 / 84

页数：6

共 25 条

[1] ESTIMATION OF NUCLEAR POPULATION FROM MICROTOME SECTIONS [J].

ABERCROMBIE, M .

ANATOMICAL RECORD, 1946, 94 (02) :239-247

[2] LONG-TERM DEVELOPMENT OF MESENCEPHALIC DOPAMINERGIC-NEURONS OF MOUSE EMBRYOS IN DISSOCIATED PRIMARY CULTURES - MORPHOLOGICAL AND HISTOCHEMICAL-CHARACTERISTICS [J].

BERGER, B ;

DIPORZIO, U ;

DAGUET, MC ;

GAY, M ;

VIGNY, A ;

GLOWINSKI, J ;

PROCHIANTZ, A .

NEUROSCIENCE, 1982, 7 (01) :193-205

[3]

BJORKLUND A, 1980, CELL TISSUE RES, V212, P39

[4]

BJORKLUND A, 1980, ACTA PHYSL SCAND S, V522, P1

[5] SELECTIVE SURVIVAL OF NEURONS FROM CHICK-EMBRYO SENSORY GANGLIONIC DISSOCIATES UTILIZING SERUM-FREE SUPPLEMENTED MEDIUM [J].

BOTTENSTEIN, JE ;

SKAPER, SD ;

VARON, SS ;

SATO, GH .

EXPERIMENTAL CELL RESEARCH, 1980, 125 (01) :183-190

[6] MONITORING OF CELL VIABILITY IN SUSPENSIONS OF EMBRYONIC CNS TISSUE AND ITS USE AS A CRITERION FOR INTRACEREBRAL GRAFT-SURVIVAL [J].

BRUNDIN, P ;

ISACSON, O ;

BJORKLUND, A .

BRAIN RESEARCH, 1985, 331 (02) :251-259

[7] RELEASE OF DOPAMINE FROM DISSOCIATED MESENCEPHALIC DOPAMINERGIC-NEURONS IN PRIMARY CULTURES IN ABSENCE OR PRESENCE OF STRIATAL TARGET-CELLS [J].

DAGUET, MC ;

DIPORZIO, U ;

PROCHIANTZ, A ;

KATO, A ;

GLOWINSKI, J .

BRAIN RESEARCH, 1980, 191 (02) :564-568

[8]

DENISDONINI S, 1983, J NEUROSCI, V3, P2292

[9] EFFECT OF STRIATAL CELLS ON INVITRO MATURATION OF MESENCEPHALIC DOPAMINERGIC-NEURONS GROWN IN SERUM-FREE CONDITIONS [J].

DIPORZIO, U ;

DAGUET, MC ;

GLOWINSKI, J ;

PROCHIANTZ, A .

NATURE, 1980, 288 (5789) :370-373

[10] FIBROUS ASTROCYTES AND REACTIVE ASTROCYTE-LIKE CELLS IN TRANSPLANTS OF CULTURED ASTROCYTE PRECURSOR CELLS [J].

DOERING, LC ;

FEDOROFF, S ;

DEVON, RM .

DEVELOPMENTAL BRAIN RESEARCH, 1983, 6 (02) :183-189

← 1 2 3 →