MICROGLIAL LOCALIZATION OF ALPHA-INTERFERON RECEPTOR IN HUMAN BRAIN-TISSUES

被引：42
|
作者：
YAMADA, T
YAMANAKA, L
机构：
[1] Department of Neurology, Chiba University, Chuo-ku, Chiba, 260, 1-8-1, Inohana
来源：
NEUROSCIENCE LETTERS | 1995年 / 189卷 / 02期
关键词：
ALPHA-INTERFERON; ALPHA-INTERFERON RECEPTOR; MICROGLIA; MACROPHAGE; HUMAN BRAIN;
D O I：
10.1016/0304-3940(95)11452-3
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The localization of the alpha-interferon receptor (alpha-IFNR) was examined in human brain tissues from neurologically normal, Alzheimer's disease (AD) and cerebral infarction cases, using one antibody (IFNaR3) which preferentially recognizes alpha 2-IFN receptor and a second antibody (anti-p40) which recognizes the soluble alpha-IFN alpha/beta receptor In all cases, microglial cells were stained with the antibodies, In infarct brains, macrophages were intensely labeled for alpha-IFNR. The anti-p40 antibody also showed capillary staining which might be due to residual blood plasma. On Western blots, IFNaR3 recognized only a 210 kDa alpha-IFN/receptor complex, indicating its localization in microglial cells. The anti-p40 antibody recognized this complex as well as four other major materials of lower molecular weight. These results suggest that alpha-IFNR protein is constitutively expressed in microglial cells.
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收藏
页码：73 / 76
页数：4
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