ATP-SENSITIVE K+-CHANNEL RUN-DOWN IS MG2+ DEPENDENT

被引：76

作者：

KOZLOWSKI, RZ ^{[1
]}

ASHFORD, MLJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT PHARMACOL, CAMBRIDGE CB2 1QJ, ENGLAND

来源：

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES | 1990年 / 240卷 / 1298期

关键词：

D O I：

10.1098/rspb.1990.0044

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

ATP- sensitive K+-channel currents were recorded from isolated membrane patches and voltage-clamped CRI-G1 insulin-secreting cells. Internal Mg2+ ions inhibited ATP-K+ channels by a voltage-dependent block of the channel current and decrease of open-state probability. The rundown of ATP-K+ channel activity was also shown to be [Mg2+](i) dependent, being almost abolished in Mg2+-free conditions. Substitution of Mn2+ for Mg2+ did not prevent run-down, nor did the presence of phosphate-donating nucleotides, a protease or phosphatase inhibitor or replacement of Cl- by gluconate.

引用

页码：397 / 410

页数：14

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