THE FLOW CYTOMETRIC ANALYSIS OF LUPIN PROTEINS' POTENTIAL TO INDUCE THE RESPIRATORY BURST IN HUMAN NEUTROPHILS

被引:0
作者
Klos, Patrycja [1 ]
Poniedzialek, Barbara [2 ]
Lampart-Szczapa, Eleonora [1 ]
Gralik, Justyna [1 ]
Wiktorowicz, Krzysztof [2 ]
机构
[1] Poznan Univ Life Sci, Poznan, Poland
[2] Univ Med Sci, Poznan, Poland
关键词
lupin globulins; immunoreactivity; respiratory burst; reactive oxygen species; neutrophils; phorbol; 12-myristate; 13-acetate;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
There is an ever-increasing demand for lupin seed protein in human nutrition. However, their usage as a food ingredient can be limited by their influence on the immune system. The aim of the study was to analyse, by means of flow cytometry, the potential of lupin seed globulins to induce the respiratory burst in human neutrophils. The results proved the potential of lupin globulins to induce the increased ROS production in human neutrophils. The increased ROS production by the cells treated with lupin globulins and, at the same time, stimulated with the PMA may suggest a synergistic effect of the globulins and the PMA.
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页码:91 / 97
页数:7
相关论文
共 20 条
[1]  
Arnoldi A., 2005, FIN C HLTH PROF 9 18, P1
[2]  
Bez J., 2005, FIN C HLTH PROF 9 18, P51
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   Frutalin, a galactose-binding lectin, induces chemotaxis and rearrangement of actin cytoskeleton in human neutrophils: Involvement of tyrosine kinase and phosphoinositide 3-kinase [J].
Brando-Lima, AC ;
Saldanha-Gama, RF ;
Henriques, MDMO ;
Monteiro-Moreira, ACO ;
Moreira, RA ;
Barja-Fidalgo, C .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2005, 208 (02) :145-154
[5]   The neutrophil: Function and regulation in innate and humoral immunity [J].
Burg, ND ;
Pillinger, MH .
CLINICAL IMMUNOLOGY, 2001, 99 (01) :7-17
[6]   Respiratory burst in human neutrophils [J].
Dahlgren, C ;
Karlsson, A .
JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 232 (1-2) :3-14
[7]   Monoclonal antibodies against the candidate lupin allergens α-conglutin and β-conglutin [J].
Dooper, Maaike M. B. W. ;
Holden, Lise ;
Faeste, Christiane K. ;
Thompson, Keith M. ;
Egaas, Eliann .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2007, 143 (01) :49-58
[8]   A case of peanut cross-allergy to lupine flour in a hot dog bread [J].
Fæste, CK ;
Lovik, M ;
Wiker, HG ;
Egaas, E .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2004, 135 (01) :36-39
[9]  
Freitas RL, 2000, INT J FOOD SCI NUTR, V51, P341, DOI 10.1080/096374800426939
[10]   Inside the neutrophil phagosome: Oxidants, myeloperoxidase, and bacterial killing [J].
Hampton, MB ;
Kettle, AJ ;
Winterbourn, CC .
BLOOD, 1998, 92 (09) :3007-3017