From the yellow petals of rape (Brassica napus), five major carotenoids have been isolated. Based on UV/VIS, CD, 400-MHz H-1-NMR, and mass spectra, they were identified as (9'Z)-neoxanthin, (all-E)- and (9Z)-violaxanthin, and (8'R)- and (8'S)-luteoxanthin.