ANALYSIS OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID SUBSTANCE, AND OF OTHER NATURAL N-ACYLETHANOLAMINES

被引：64
作者：
FONTANA, A
DIMARZO, V
CADAS, H
PIOMELLI, D
机构：
[1] CNR,IST CHIM MOLEC INTERESSE BIOL,I-80072 ARCO,ITALY
[2] INSERM,U109,F-75014 PARIS,FRANCE
来源：
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1995年 / 53卷 / 04期
关键词：
D O I：
10.1016/0952-3278(95)90130-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquid-phase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.
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页码：301 / 308
页数：8
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