A SMALL, HIGH-COPY-NUMBER VECTOR SUITABLE FOR BOTH IN-VITRO AND IN-VIVO GENE-EXPRESSION

被引：5
作者：
HUANG, BH
SHI, ZT
TSAI, MD
机构：
[1] OHIO STATE UNIV, DEPT CHEM, COLUMBUS, OH 43210 USA
[2] OHIO STATE UNIV, OHIO STATE BIOCHEM PROGRAM, COLUMBUS, OH 43210 USA
来源：
GENE | 1994年 / 151卷 / 1-2期
关键词：
CLONING; COUPLED TRANSCRIPTION TRANSLATION SYSTEM OF ZUBAY; ESCHERICHIA COLI; PHOSPHOLIPASE AZ; ADENYLATE KINASE; CHICKEN;
D O I：
10.1016/0378-1119(94)90645-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A 2433-bp, high-copy-number expression vector, pUK, was constructed from pUC19 and pKK223-3. The genes encoding chicken adenylate kinase (AK) and phospholipase A(2), cloned into pUK, were highly expressed in vitro using the coupled transcription/translation system of Zubay. High-level in vivo expression in Escherichia coli was also demonstrated for the AK gene cloned in this vector. The multiple cloning site (MCS) of pKK223-3 was preserved in pUK. In addition, three more sites in the MCS sequence, AccI, BamHI and SalI, were made unique for the convenience of cloning.
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页码：143 / 145
页数：3
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