QUANTITATIVE MEASUREMENT OF HUMAN INTERLEUKIN-2 RECEPTOR LEVELS WITH INTACT AND DETERGENT-SOLUBILIZED HUMAN T-CELLS

被引：25

作者：

DOWER, SK ^{[1
]}

HEFENEIDER, SH ^{[1
]}

ALPERT, AR ^{[1
]}

URDAL, DL ^{[1
]}

机构：

[1] IMMUNEX CORP, 51 UNIV ST, SEATTLE, WA 98105 USA

来源：

MOLECULAR IMMUNOLOGY | 1985年 / 22卷 / 08期

关键词：

D O I：

10.1016/0161-5890(85)90080-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2A3 monoclonal antibody (.gamma.1, .kappa.) is a novel high-affinity reagent for detecting the human interleukin 2 (IL-2) receptor. The antibody inhibits IL-2 binding to its receptor and is an antagonist of IL-2 action. Detailed analysis of the mechanism of binding of the IgG, (Fab'')2 and Fab'' of 2A3 antibody shows that the bivalent species cross-link on the cell surface when bound. Measurements of IL-2 receptor expression on digitonin-permeabilized cells suggest that the intracellular pool of receptors is small. The antibody will bind to IL-2 receptors on glutaraldehyde-fixed cells in the presence of Triton X-100. This property is used in designing an assay for quantitative measurements of IL-2 receptor concentration in solution. This assay can be used to monitor receptor protein during purification to homogeneity.

引用

页码：937 / 947

页数：11

共 44 条

[1]

BUBBERS JE, 1975, J IMMUNOL, V114, P1126

[2] TRANSIENT EXPRESSION OF INTERLEUKIN-2 RECEPTORS - CONSEQUENCES FOR T-CELL GROWTH [J].