QUANTITATIVE MEASUREMENT OF HUMAN INTERLEUKIN-2 RECEPTOR LEVELS WITH INTACT AND DETERGENT-SOLUBILIZED HUMAN T-CELLS

被引:25
作者
DOWER, SK [1 ]
HEFENEIDER, SH [1 ]
ALPERT, AR [1 ]
URDAL, DL [1 ]
机构
[1] IMMUNEX CORP, 51 UNIV ST, SEATTLE, WA 98105 USA
关键词
D O I
10.1016/0161-5890(85)90080-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
2A3 monoclonal antibody (.gamma.1, .kappa.) is a novel high-affinity reagent for detecting the human interleukin 2 (IL-2) receptor. The antibody inhibits IL-2 binding to its receptor and is an antagonist of IL-2 action. Detailed analysis of the mechanism of binding of the IgG, (Fab'')2 and Fab'' of 2A3 antibody shows that the bivalent species cross-link on the cell surface when bound. Measurements of IL-2 receptor expression on digitonin-permeabilized cells suggest that the intracellular pool of receptors is small. The antibody will bind to IL-2 receptors on glutaraldehyde-fixed cells in the presence of Triton X-100. This property is used in designing an assay for quantitative measurements of IL-2 receptor concentration in solution. This assay can be used to monitor receptor protein during purification to homogeneity.
引用
收藏
页码:937 / 947
页数:11
相关论文
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