APPARENTLY IRREVERSIBLE GTP HYDROLYSIS ATTENDS TUBULIN SELF-ASSEMBLY

被引：10

作者：

ANGELASTRO, JM ^{[1
]}

PURICH, DL ^{[1
]}

机构：

[1] UNIV FLORIDA, J HILLIS MILLER HLTH CTR, DEPT BIOCHEM & MOLEC BIOL, BOX J-245, GAINESVILLE, FL 32610 USA

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 191卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb19150.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pathway of GTP hydrolysis associated with microtubule polymerization was investigated using an assay of intermediate 18O‐exchange reactions. Under a variety of conditions influencing tubulin self‐assembly, GTP was hydrolyzed without any evidence of multiple reversals characteristic of reversible phosphoanhydride‐bond cleavage. These results also accord with published findings that ATP hydrolysis during actin polymerization fails to display intermediate exchange reactions [Carlier, M. F., Pantaloni, D., Evans, J. A., Lambooy, P. K., Korn, E. D. and Webb, M. R. (1988) FEBS Lett. 235, 211–214]. Copyright © 1990, Wiley Blackwell. All rights reserved

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页码：507 / 511

页数：5

相关论文

共 25 条

[1] ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].

BENSADOUN, A ;

WEINSTEIN, D .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :241-250

[2] INTERACTIONS OF TUBULIN WITH VINBLASTINE AND GUANOSINE TRIPHOSPHATE [J].

BERRY, RW ;

SHELANSKI, ML .

JOURNAL OF MOLECULAR BIOLOGY, 1972, 71 (01) :71-+

[3] MECHANISM OF GTP HYDROLYSIS IN TUBULIN POLYMERIZATION - CHARACTERIZATION OF THE KINETIC INTERMEDIATE MICROTUBULE-GDP-PI USING PHOSPHATE ANALOGS [J].

CARLIER, MF ;

DIDRY, D ;

SIMON, C ;

PANTALONI, D .

BIOCHEMISTRY, 1989, 28 (04) :1783-1791

[4] STABILIZATION OF MICROTUBULES BY INORGANIC-PHOSPHATE AND ITS STRUCTURAL ANALOGS, THE FLUORIDE COMPLEXES OF ALUMINUM AND BERYLLIUM [J].

CARLIER, MF ;

DIDRY, D ;

MELKI, R ;

CHABRE, M ;

PANTALONI, D .

BIOCHEMISTRY, 1988, 27 (10) :3555-3559

[5] THE HYDROLYSIS OF ATP THAT ACCOMPANIES ACTIN POLYMERIZATION IS ESSENTIALLY IRREVERSIBLE [J].

CARLIER, MF ;

PANTALONI, D ;

EVANS, JA ;

LAMBOOY, PK ;

KORN, ED ;

WEBB, MR .

FEBS LETTERS, 1988, 235 (1-2) :211-214

[6] KINETIC-ANALYSIS OF GUANOSINE 5'-TRIPHOSPHATE HYDROLYSIS ASSOCIATED WITH TUBULIN POLYMERIZATION [J].

CARLIER, MF ;

PANTALONI, D .

BIOCHEMISTRY, 1981, 20 (07) :1918-1924

[7] PREPARATIVE SEPARATION OF NUCLEOTIDES BY HIGH-PERFORMANCE-ION EXCHANGE LIQUID-CHROMATOGRAPHY USING A VOLATILE BUFFER SYSTEM [J].

GREENHUT, J ;

RUDOLPH, FB .

JOURNAL OF CHROMATOGRAPHY, 1985, 319 (03) :461-466

[8] PARTICIPATION OF GUANINE-NUCLEOTIDES IN NUCLEATION AND ELONGATION STEPS OF MICROTUBULE ASSEMBLY [J].

KARR, TL ;

PODRASKY, AE ;

PURICH, DL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (11) :5475-5479

[9]

KIRSCHNER MW, 1989, HARVEY LECT, V83, P1

[10]

KOBAYASHI T, 1975, J BIOCHEM-TOKYO, V77, P1193