OPIOID-PEPTIDES DETERMINATION BY TYROSINASE-MODIFIED OXYGEN-ELECTRODE

被引：5

作者：

EREMENKO, AV

BARMIN, AV

KUROCHKIN, IN

机构：

[1] Department of Biosensors, Research Center for Molecular Diagnostics & Therapy, 113149, Moscow

来源：

ANALYTICAL LETTERS | 1995年 / 28卷 / 13期

关键词：

TYROSINASE; OPIOID PEPTIDES; ENZYME ELECTRODE; AMPEROMETRY;

D O I：

10.1080/00032719508000373

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Opioid peptides morphiceplin (Tyr-Pro-Phe-Pro-NH2), [D-Ala(2), D-Leu(5)]-enkephalin (Tyr-D-Ala-Gly-Phe-D-Leu, DADLE) and [D-Thr(2)]-Leu-enkephalin-Thr (Tyr-D-Thr-Gly-Phe-Leu-Thr, DTLET) containing tyrosine has been studied in the reaction with mushroom tyrosinase immobilized on Clark-type oxygen electrode. A 4-6-times higher response is observed for tyrosine compared to peptides. The detection Limit of the tyrosinase-modified electrode for DADLE, morphiceptin and DTLET was 6 mu M, 9 mu M and 16 mu M, respectively.

引用

页码：2297 / 2304

页数：8

共 14 条

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[2]

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[3]

IBORRA JL, 1981, BIOCHEM EDUC, V9, P51

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