ISOLATION AND CHARACTERIZATION OF TEMPERATURE-SENSITIVE PLC1 MUTANTS OF THE YEAST SACCHAROMYCES-CEREVISIAE

被引：26
作者：
YOKOO, T
KATO, H
MATSUI, Y
TAKENAWA, T
TOHE, A
机构：
[1] UNIV TOKYO,GRAD SCH SCI,DEPT PLANT SCI,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,INST MED SCI,DEPT MOLEC ONCOL,MINATO KU,TOKYO 108,JAPAN
来源：
MOLECULAR & GENERAL GENETICS | 1995年 / 247卷 / 02期
关键词：
SACCHAROMYCES CEREVISIAE; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C; TEMPERATURE-SENSITIVE MUTANT; PLC1;
D O I：
10.1007/BF00705644
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The PLC1 gene of the yeast Saccharomyces cerevisiae has been discovered to encode a homolog of mammalian phosphoinositide-specific phospholipase C (PLC). Five temperature-sensitive plc1 mutants were isolated by in vitro mutagenesis with subsequent plasmid shuffling. All of the amino acid substitutions that caused a temperature-sensitive growth phenotype were located in the X or the Y region, both of which are conserved among PLC isoenzymes. The PLC activity of all products of mutant plc1 genes was dramatically lower than that of the wild-type product, indicating that PLC activity itself is important for cell growth. At the restrictive temperature, plc1 mutant cells ceased growth at random times during the cell cycle, a result that suggests that PLC1 is required at several or all stages of the cell cycle.
引用
收藏
页码：148 / 156
页数：9
相关论文
共 50 条
[21]   Characterization of a temperature-sensitive mutation that impairs the function of yeast tRNA nucleotidyltransferase [J].
Shan, Xunying ;
Russell, Tara A. ;
Paul, Scott M. ;
Kushner, David B. ;
Joyce, Paul B. M. .
YEAST, 2008, 25 (03) :219-233
[22]   Isolation of Fission Yeast Condensin Temperature-Sensitive Mutants with Single Amino Acid Substitutions Targeted to Hinge Domain [J].
Xu, Xingya ;
Yanagida, Mitsuhiro .
G3-GENES GENOMES GENETICS, 2019, 9 (05) :1777-1783
[23]   Maximum glucoamylase production by a temperature-sensitive mutant of Saccharomyces cerevisiae in batch culture [J].
Lee, DH ;
Uchiyama, K ;
Shioya, S ;
Hwang, YI .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 20 (3-4) :160-165
[24]   CDKA and CDKB kinases from Chlamydomonas reinhardtii are able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae [J].
Cizkova, M. ;
Pichova, A. ;
Vitova, M. ;
Hlavova, M. ;
Hendrychova, J. ;
Umysova, D. ;
Galova, E. ;
Sevovicova, A. ;
Zachleder, V. ;
Bisova, K. .
PROTOPLASMA, 2008, 232 (3-4) :183-191
[25]   The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H+-ATPase [J].
Coccetti, P ;
Tisi, R ;
Martegani, E ;
Teixeira, LS ;
Brandao, RL ;
de Miranda Castro, I ;
Thevelein, JM .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1405 (1-3) :147-154
[26]   Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus [J].
Matsuo, M ;
Kurokawa, K ;
Nishida, S ;
Lu, Y ;
Takimura, H ;
Kaito, C ;
Fukuhara, N ;
Maki, H ;
Miura, K ;
Murakami, K ;
Sekimizu, K .
FEMS MICROBIOLOGY LETTERS, 2003, 222 (01) :107-113
[27]   Isolation and characterization of a high-acetate-producing sake yeast Saccharomyces cerevisiae [J].
Kurita, O ;
Nakabayashi, T ;
Saitho, K .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2003, 95 (01) :65-71
[28]   CHARACTERIZATION OF THE CYR1-2 UGA MUTATION IN SACCHAROMYCES-CEREVISIAE [J].
MORISHITA, T ;
MATSUURA, A ;
UNO, I .
MOLECULAR & GENERAL GENETICS, 1993, 237 (03) :463-466
[29]   ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF BOMBYX-MORI NUCLEAR POLYHEDROSIS-VIRUS [J].
HASHIMOTO, Y ;
SOGA, T ;
YANASE, T ;
MATSUMOTO, T .
JAPANESE JOURNAL OF APPLIED ENTOMOLOGY AND ZOOLOGY, 1995, 39 (01) :33-37
[30]   ISOLATION AND CHARACTERIZATION OF A MULTIENZYME COMPLEX CONTAINING DNA REPLICATIVE ENZYMES FROM MITOCHONDRIA OF SACCHAROMYCES-CEREVISIAE - MULTIENZYME COMPLEX FROM YEAST MITOCHONDRIA [J].
MURTHY, V ;
PASUPATHY, K .
MOLECULAR BIOLOGY REPORTS, 1995, 20 (03) :135-141
12345