DETERMINATION OF MESOCARB METABOLITES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION AND WITH MASS-SPECTROMETRY USING A PARTICLE-BEAM INTERFACE

被引：18
|
作者：
VENTURA, R
NADAL, T
ALCALDE, P
SEGURA, J
机构：
[1] Departament de Farmacologia i Toxicologia, Institut Municipal d'Investigació Mèdica, Autonomous University of Barcelona, 08003 Barcelona
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 647卷 / 02期
关键词：
D O I：
10.1016/0021-9673(93)83402-E
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method of screening for mesocarb ingestion in doping control is described. After alkaline extraction with ethyl acetate, samples are analysed by reversed-phase high-performance liquid chromatography (HPLC) with UV detection. A peak with a shorter retention time than and a UV spectrum identical with those of unchanged mesocarb was obtained when positive urine extracts were analysed. The metabolite was identified as the sulphate conjugate of p-hydroxymesocarb after HPLC-mass spectrometry with a particle-beam interface and hydrolysis studies. The compound was detected in urine until 48-72 h after administration of single doses of 10 mg. Unchanged mesocarb and free p-hydroxymesocarb were not detected in the samples studied.
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页码：203 / 210
页数：8
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