GLOBAL TARGET ANALYSIS OF FLUORESCENCE DEPOLARIZATION IN MODEL MEMBRANES USING EXPONENTIAL SPLINES

被引：18
|
作者：
ARCIONI, A
TARRONI, R
ZANNONI, C
机构：
来源：
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1993年 / 89卷 / 15期
关键词：
D O I：
10.1039/ft9938902815
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
A generalization of the global target deconvolution procedure for the analysis of fluorescence depolarization data in membrane vesicles is proposed. This new procedure links data at different temperatures using exponential spline interpolating functions and is suitable for systems that present phase transitions. Here we have performed and analysed single-photon measurements for the probe 1,6-diphenylhexatriene (DPH) embedded in dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and distearoylphosphatidylcholine (DSPC) multilamellar vesicles, obtaining order parameter [P2] and rotational diffusion coefficient D(perpendicular-to) as a function of temperature.
引用
收藏
页码：2815 / 2822
页数：8
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