EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-2A BLOCKS CALCIUM MOBILIZATION IN B-LYMPHOCYTES

被引：118
作者：
MILLER, CL
LONGNECKER, R
KIEFF, E
机构：
[1] HARVARD UNIV,SCH MED,DEPT MED,75 FRANCIS ST,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115
来源：
JOURNAL OF VIROLOGY | 1993年 / 67卷 / 06期
关键词：
D O I：
10.1128/JVI.67.6.3087-3094.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
LMP2A is expressed in latent Epstein-Barr virus (EBV) infection and interacts with LMP1 and members of the src tyrosine kinase family in the plasma membrane. Since tyrosine kinases mediate receptor-induced changes in intracellular free calcium, the effect of LMP2A on receptor-mediated intracellular calcium mobilization was evaluated by stably expressing LMP2A in an EBV-negative Burkitt tumor cell line (BJAB) or in LMP1-converted BJAB cells. LMP2A significantly blocked calcium mobilization following class II, CD19, or immunoglobulin M cross-linking. LMP2A effects were partially reversed in LMP1-converted cell lines. These results are compatible with LMP2A acting in latent B-lymphocyte infection to downmodulate LMP1 effects on cell growth or to inhibit induction of lytic EBV infection in specific human tissues following receptor ligation.
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页码：3087 / 3094
页数：8
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