Molecular detection of Cryptosporidium species in street-sampled dog faeces in Ibadan, Nigeria

Cryptosporidium, an intestinal protozoan parasite that infects a wide range of animals, is shed to the environment through the faeces of infected hosts that include humans and companion animals. Environmental contamination with infective oocysts increases the risk of transmission of this zoonotic pathogen to other susceptible host. In this study, Cryptosporidium was detected by polymerase chain reaction amplification of the 18S rRNA gene in 2.5% (5/203) of dog faecal samples randomly collected from 23 streets of Ibadan, southwestern Nigeria. Nucleotide sequencing of the 18S rRNA gene yielded two genotypes, C. parvum and C. muris in three and two samples, respectively. Further amplification of the gp60 gene-coding 60-kDa glycoprotein for subtype determination detected two isolates as C. parvum subtype IIc family. C. parvum subtype IIc is a human-derived pathogen while C. muris has been reported to infect rodents and even humans. The findings of this study suggest that free-ranging urban dogs could act as reservoirs and potential sources of environmental contamination with Cryptosporidium species of public health importance.