Practical aspects of leukotriene E(4) assay in the urine

被引:0
作者
Chabane, MH [1 ]
LeVraux, V [1 ]
LeProvost, AM [1 ]
Leynadier, F [1 ]
机构
[1] STALLERGENES SA,FRESNES,FRANCE
关键词
leukotriene E(4); immunoessay; enzyme; allergy; inflammation;
D O I
暂无
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Leukotrienes constitute a class of potent biological mediators of inflammation arid anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and radioimmunological capabilities. The recent availability of kits for immunoenzymatic assay of leukotrienes offers a new tools for clinical laboratories provided the limitations of the method are understood. We suggest a simplified methodology for direct urinary LTE(4) detection and outline a number of areas of concern encountered with this method.
引用
收藏
页码:543 / 548
页数:6
相关论文
共 26 条
  • [1] DEVELOPMENT OF ENZYME IMMUNOASSAYS FOR LEUKOTRIENES USING ACETYLCHOLINESTERASE
    ANTOINE, C
    LELLOUCHE, JP
    MACLOUF, J
    PRADELLES, P
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1075 (02) : 162 - 168
  • [2] THE EFFECTS OF INHALED LEUKOTRIENE E4 ON THE AIRWAY RESPONSIVENESS TO HISTAMINE IN SUBJECTS WITH ASTHMA AND NORMAL SUBJECTS
    ARM, JP
    SPUR, BW
    LEE, TH
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1988, 82 (04) : 654 - 660
  • [3] AUTEGARDEN JE, 1994, PRESSE MED, V23, P1181
  • [4] DETERMINATION OF SULFIDOPEPTIDE LEUKOTRIENES IN BIOLOGICAL-FLUIDS BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
    BALAZY, M
    MURPHY, RC
    [J]. ANALYTICAL CHEMISTRY, 1986, 58 (06) : 1098 - 1101
  • [5] CHARACTERIZATION OF HUMAN AIRWAY EPITHELIAL-CELL LEUKOTRIENE A(4) HYDROLASE
    BIGBY, TD
    LEE, DM
    MINAMI, M
    OHISHI, N
    SHIMIZU, T
    BAKER, JR
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1994, 11 (05) : 615 - 624
  • [6] CHRISTIE PE, 1994, EUR RESPIR J, V7, P907
  • [7] DAVIDSON D, 1995, AMJ RESPIR CRIT CARE, V11, P841
  • [8] FELSIEN D, 1991, AM REV RESPIR DIS, V143, P1322
  • [9] THE ROLE OF LEUKOTRIENES IN INFLAMMATION
    HENDERSON, WR
    [J]. ANNALS OF INTERNAL MEDICINE, 1994, 121 (09) : 684 - 697
  • [10] THE MECHANISM OF LTE4-INDUCED HISTAMINE HYPERRESPONSIVENESS IN GUINEA-PIG TRACHEAL AND HUMAN BRONCHIAL SMOOTH-MUSCLE, INVITRO
    JACQUES, CAJ
    SPUR, BW
    JOHNSON, M
    LEE, TH
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1991, 104 (04) : 859 - 866