CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE NADP-SPECIFIC GLUTAMATE-DEHYDROGENASE FROM NEUROSPORA-CRASSA

被引：4
作者：
STILLMAN, TJ
YIP, KSP
RICE, DW
FUENTES, AM
CONNERTON, I
机构：
[1] UNIV SHEFFIELD,KREBS INST BIOMOLEC RES,DEPT MOLEC BIOL & BIOTECHNOL,SHEFFIELD S10 2UH,S YORKSHIRE,ENGLAND
[2] BBSRC,INST FOOD RES,DEPT PROT ENGN,READING RG6 2EF,BERKS,ENGLAND
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1995年 / 51卷
关键词：
D O I：
10.1107/S0907444995000904
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The NADP-linked glutamate dehydrogenase from Neurospora crassa has been crystallized by the hanging-drop method of vapour diffusion in the presence of 0.1 M glutamate. The crystals are trigonal and are in space group P3(1)21 with unit-cell dimensions of a = b = 196.6, c = 102.0 Angstrom and with a trimer in the asymmetric unit. A full structure determination of this enzyme will lead to an understanding of the molecular basis of inter-allelic complementation observed with hybrid hexamers of naturally occurring mutants.
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页码：837 / 839
页数：3
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