CHEMILUMINESCENCE INTENSITIES AND SPECTRA OF LUMINOL OXIDATION BY SODIUM-HYPOCHLORITE IN THE PRESENCE OF HYDROGEN-PEROXIDE

被引：41

作者：

ARNHOLD, J

MUELLER, S

ARNOLD, K

GRIMM, E

机构：

[1] Institute of Biophysics, School of Medicine, University of Leipzig

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1991年 / 6卷 / 03期

关键词：

LUMINOL; SODIUM HYPOCHLORITE; HYDROGEN PEROXIDE; CHEMILUMINESCENCE INTENSITIES; CHEMILUMINESCENCE SPECTRA;

D O I：

10.1002/bio.1170060309

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen peroxide amplifies the chemiluminescence in the odixation of luminol by sodium hypochlorite. A linear relationship between concentration of hydrogen peroxide and light intensity was found in the concentration range 5 x 10(-8)-7.5 x 10(-6) mol/l. At 7.5 x 10(-6) mol/l H2O2 the chemiluminescence is amplified 550-fold. The chemiluminescence spectra of these reactions have a wavelength maximum at 431 nm independent of the concentration of hydrogen peroxide. The results indicate that hydrogen peroxide is a necessary component in the chemiluminescent oxidation of the luminol by sodium hypochlorite.

引用

页码：189 / 192

页数：4

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