PURIFICATION AND SOME PROPERTIES OF BETA-MANNANASE FROM BACILLUS SP

被引：21
作者：
OOI, T
KIKUCHI, D
机构：
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1995年 / 11卷 / 03期
关键词：
BACILLUS SP; MANNAN; BETA-MANNANASE;
D O I：
10.1007/BF00367106
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-Mannanase produced by Bacillus sp. W-2, isolated from decayed commercial konjak cake, was purified from the culture supernatant by (NH4)(2)SO4 precipitation, adsorption to konjak gel, and column chromatography with DEAE-cellulose, Sephadex G-100 and Sephacryl S-200. Its molecular size was estimated by SDS-PAGE as 40 kDa, and by gel filtration as 36 kDa. The enzyme was most active at pH 7 and 70 degrees C and was stable for at least 1 h between pH 5 and 10 and below 60 degrees C. Its activity was completely inhibited by Hg2+. The enzyme hydrolysed galactomannan better than glucomannan and mainly produced mannose and mannobiose.
引用
收藏
页码：310 / 314
页数：5
相关论文
共 50 条
[31]   Purification and characteristics of chitosanase from Bacillus sp HW-002 [J].
Lee, HW ;
Choi, JW ;
Han, DP ;
Park, MJ ;
Lee, NW ;
Yi, DH .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 6 (01) :19-25
[32]   Purification and characterization of a novel β-galactosidase from Bacillus sp MTCC 3088 [J].
Chakraborti, S ;
Sani, RK ;
Banerjee, UC ;
Sobti, RC .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2000, 24 (01) :58-63
[33]   Effects of Supplemental Beta-mannanase on Digestible Energy and Metabolizable Energy Contents of Copra Expellers and Palm Kernel Expellers Fed to Pigs [J].
Kwon, W. B. ;
Kim, B. G. .
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2015, 28 (07) :1014-1019
[34]   Fermenter scale production of recombinant beta-mannanase by E. coli BL21 cells under microaerobic environment [J].
Purohit, Anjali ;
Pawar, Lata ;
Yadav, Sudesh Kumar .
CARBOHYDRATE RESEARCH, 2024, 541
[35]   Properties of an amylase from thermophilic Bacillus sp. [J].
de Carvalho, Raquel Vieira ;
Ribeiro Correa, Thamy Livia ;
Matos da Silva, Julia Caroline ;
Coutinho de Oliveira Mansur, Luciana Ribeiro ;
Leal Martins, Meire Lelis .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2008, 39 (01) :102-107
[36]   Purification and Characterization of Carboxymethyl Cellulase from Bacillus sp Isolated from a Paddy Field [J].
Vijayaraghavan, Ponnuswamy ;
Vincent, S. G. Prakash .
POLISH JOURNAL OF MICROBIOLOGY, 2012, 61 (01) :51-55
[37]   Cloning, purification, and characterization of chitinase from Bacillus sp DAU101 [J].
Lee, Yong-Seok ;
Park, In-Hye ;
Yoo, Ju-Soon ;
Chung, Soo-Yeol ;
Lee, Young-Choon ;
Cho, Young-Su ;
Ahn, Soon-Cheol ;
Kim, Cheol-Min ;
Choi, Yong-Lark .
BIORESOURCE TECHNOLOGY, 2007, 98 (14) :2734-2741
[38]   Purification and characterization of xylanase from Bacillus sp. strain DSNC 101 [J].
Cho, MC ;
Bai, S .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 7 (06) :386-390
[39]   Purification and Characterization of Thermostable α-Amylase from Soil Bacterium Bacillus sp. [J].
Enez, Baris .
PROTEIN AND PEPTIDE LETTERS, 2021, 28 (12) :1372-1378
[40]   COST-EFFECTIVE ENDO-MANNANASE FROM Bacillus sp CFR1601 AND ITS APPLICATION IN GENERATION OF OLIGOSACCHARIDES FROM GUAR GUM AND AS DETERGENT ADDITIVE [J].
Srivastava, Praveen Kumar ;
Kapoor, Mukesh .
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2014, 44 (04) :392-417
12345