THE STREPTOKINASE DOMAIN RESPONSIBLE FOR PLASMINOGEN BINDING

被引：23
作者：
RODRIGUEZ, P
FUENTES, D
MUNOZ, E
RIVERO, D
ORTA, D
ALBURQUERQUE, S
PEREZ, S
BESADA, V
HERRERA, L
机构：
[1] CTR GENET ENGN & BIOTECHNOL,DIV THROMBOYLT,HAVANA,CUBA
[2] CTR GENET ENGN & BIOTECHNOL,DIV PHYS CHEM,HAVANA,CUBA
来源：
FIBRINOLYSIS | 1994年 / 8卷 / 05期
关键词：
D O I：
10.1016/0268-9499(94)90016-7
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The limited proteolysis of soluble recombinant streptokinase by Sepharose-immobilized human plasmin(ogen) complexed with recombinant streptokinase is described. A 17kDa streptokinase fragment comprising residues Val143 (Glu148) to Arg289 (Lys293) has been shown to be the smallest stable plasminogen binding domain. Moreover, this region seems also to be important for plasminogen activation.
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页码：276 / 285
页数：10
相关论文
共 34 条
[11]  
FERNANDEZPATRON C, 1992, BIOTECHNIQUES, V12, P564
[12]   STUDY OF PROTEASES AND PROTEASE-INHIBITOR COMPLEXES IN BIOLOGICAL-FLUIDS [J].
GRANELLIPIPERNO, A ;
REICH, E .
JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 148 (01) :223-234
[13]   ACTIVE STREPTOKINASE FROM THE CLONED GENE IN STREPTOCOCCUS-SANGUIS IS WITHOUT THE CARBOXYL-TERMINAL-32 RESIDUES [J].
JACKSON, KW ;
MALKE, H ;
GERLACH, D ;
FERRETTI, JJ ;
TANG, J .
BIOCHEMISTRY, 1986, 25 (01) :108-114
[14]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[15]   SITE-SPECIFIC ALTERATION OF GLY-24 IN STREPTOKINASE - ITS EFFECT ON PLASMINOGEN ACTIVATION [J].
LEE, BR ;
PARK, SK ;
KIM, JH ;
BYUN, SM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1085-1090
[16]   TWO-DIMENSIONAL H-1-NMR STUDIES OF THE SOLUTION STRUCTURE OF PLASMINOGEN KRINGLE-4 [J].
MABBUTT, BC ;
WILLIAMS, RJP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 170 (03) :539-548
[17]   EXPRESSION OF A STREPTOKINASE GENE FROM STREPTOCOCCUS-EQUISIMILIS IN STREPTOCOCCUS-SANGUIS [J].
MALKE, H ;
GERLACH, D ;
KOHLER, W ;
FERRETTI, JJ .
MOLECULAR AND GENERAL GENETICS, 1984, 196 (02) :360-363
[18]  
MARKUS G, 1976, J BIOL CHEM, V251, P6495
[19]   MECHANISM OF ACTIVATION OF HUMAN PLASMINOGEN BY STREPTOKINASE [J].
MCCLINTOCK, DK ;
BELL, PH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 43 (03) :694-+
[20]   CONSTRUCTION, EXPRESSION, AND PURIFICATION OF RECOMBINANT KRINGLE-1 OF HUMAN PLASMINOGEN AND ANALYSIS OF ITS INTERACTION WITH OMEGA-AMINO ACIDS [J].
MENHART, N ;
SEHL, LC ;
KELLEY, RF ;
CASTELLINO, FJ .
BIOCHEMISTRY, 1991, 30 (07) :1948-1957
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