Growth Hormone Increases Beta-Cell Proliferation in Transplanted Human and Fetal Rat Islets

被引:0
作者
Hoglund, Erika [1 ]
Mattsson, Goran [1 ]
Tyrberg, Bjorn [1 ]
Andersson, Arne [1 ]
Carlsson, Carina [1 ]
机构
[1] Uppsala Univ, Dept Med Cell Biol, S-75123 Uppsala, Sweden
来源
JOURNAL OF THE PANCREAS | 2009年 / 10卷 / 03期
基金
瑞典研究理事会;
关键词
Cell Proliferation; Human Growth Hormone; Insulin-Secreting Cells; Islets of Langerhans; Transplantation;
D O I
暂无
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Objective The aim of the study was to increase the number of human islet beta-cells after transplantation with injections of human growth hormone (hGH). Interventions Human islets and fetal rat islets were transplanted under the left kidney capsule and under the right kidney capsule, respectively in nude normoglycemic mice which were then given a daily injection of 200 mu g hGH for 1-4 weeks. Main outcome measure Beta-cell proliferation was determined using thymidine incorporation and the beta-cell area was assessed using light microscopy. Results Mice given hGH increased their body weight one week after transplantation and had a more efficient removal of glucose after 3 and 4 weeks. Treatment with hGH resulted in increased beta-cell proliferation in human and fetal rat beta-cells, and the beta-cell area tended to increase. However, serum insulin concentrations and pancreas insulin content remained unchanged. Conclusions hGH increased the proliferation of transplanted human beta-cells as well as improving the glucose tolerance of the transplanted mice.
引用
收藏
页码:242 / 248
页数:7
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