On-line coupling of isolation in situ concentration integrated with derivative synchronous spectrofluorimetry for the simultaneous determination of epinephrine and norepinepluine in urine

被引:47
作者
Canizares, P [1 ]
deCastro, MDL [1 ]
机构
[1] UNIV CORDOBA,FAC SCI,DEPT ANALYT CHEM,E-14004 CORDOBA,SPAIN
关键词
fluorimetry; flow system; urine; epinephrine; norepinephrine; isolation in situ concentration;
D O I
10.1016/0003-2670(95)00421-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An unsegmented now system for the automated determination of epinephrine and norepinephrine in urine is proposed. The simultaneous determination involves the following on-line steps: (i) matrix isolation/analyte preconcentration in an exchange minicolumn located in the loop of a injection valve; (ii) elution-injection into the dynamic system and derivatization during the transport to the detector; (iii) in situ concentration in the now cell integrated with the synchronous spectrofluorimetric scan; (iv) data acquisition and derivation by computer. The method thus developed enables a linear determination range between 2 and 60 ng ml(-1) for both catecholamines with R.S.D.s of 1.0 and 2.0% for norepinephrine and epinephrine, respectively, and a sample throughput of 12 samples h(-1). The utility of the method has been proved by applying it to urine samples with low and high concentrations of the target analytes.
引用
收藏
页码:335 / 341
页数:7
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