BINDING OF RIBULOSE 1,5-BISPHOSPHATE TO THE NONCATALYTIC SITES OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE AND ITS METABOLIC IMPLICATIONS

被引：0
作者：
YOKOTA, A
HIGASHIOKA, M
TAIRA, T
USUDA, H
WADANO, A
MURAYAMA, H
机构：
[1] UNIV OSAKA PREFECTURE,DEPT HORT & AGRON,SAKAI,OSAKA 593,JAPAN
[2] UNIV OSAKA PREFECTURE,DEPT AGR CHEM,SAKAI,OSAKA 593,JAPAN
[3] TEIKYO UNIV,FAC MED,CHEM LAB,HACHIOJI,TOKYO 19203,JAPAN
来源：
PLANT AND CELL PHYSIOLOGY | 1994年 / 35卷 / 02期
关键词：
NONCATALYTIC SUBSTRATE BINDING SITES; RAPHANUS-SATIVUS; RIBULOSE 1,5-BISPHOSPHATE; RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE; SPINACEA-OLERACEA;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
This is the first report showing that ribulose bisphosphate carboxylase/oxygenase has the non-catalytic sites to bind ribulose bisphosphate (RuBP). A plot of the binding number against the RuBP concentration in the equilibrium binding assay gave a bumpy curve with an intermediate plateau at 0.3 to 0.5 mM RuBP. The binding was saturated with 1.5 mM RuBP. The concentrations of free and binding forms of RuBP and the functioning forms of the enzyme in chloroplasts could be predicted using the kinetic data of the binding.
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页码：317 / 321
页数：5
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