GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION

被引：5105
作者：
CHALFIE, M
TU, Y
EUSKIRCHEN, G
WARD, WW
PRASHER, DC
机构：
[1] RUTGERS STATE UNIV,COOK COLL,DEPT BIOCHEM & MICROBIOL,NEW BRUNSWICK,NJ 08903
[2] WOODS HOLE OCEANOG INST,DEPT BIOL,WOODS HOLE,MA 02543
来源：
SCIENCE | 1994年 / 263卷 / 5148期
关键词：
D O I：
10.1126/science.8303295
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
引用
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页码：802 / 805
页数：4
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