HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF TETRAHYDRO-BETA-CARBOLINES IN FOOD PLANTS

被引：11

作者：

TSUCHIYA, H ^{[1
]}

YAMADA, K ^{[1
]}

KATO, H ^{[1
]}

HAYASHI, H ^{[1
]}

MIYAZAKI, T ^{[1
]}

HAYASHI, T ^{[1
]}

机构：

[1] GRELAN PHARMACEUTICAL CO LTD,TOKYO 205,JAPAN

来源：

PHYTOCHEMICAL ANALYSIS | 1995年 / 6卷 / 06期

关键词：

TETRAHYDRO-BETA-CARBOLINES; FOOD PLANTS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; ARTIFACT INTERFERENCE;

D O I：

10.1002/pca.2800060605

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is reported for the unambiguous determination of the amount of tetrahydro-beta-carbolines in food plants. In the method, plant homogenates, together with an internal standard, were first reacted with fluorescamine, serially extracted, and then subjected to reversed-phase ion-pair chromatography with fluorometric detection. The pre-treatment inhibited the artefactual formation of tetrahydro-beta-carbolines during analysis by removing the precursor tryptamine from the analytical system. Only the original 1,2,3,4-tetrahydro-beta-carboline and 1-methyl-1,2,3,4-tetrahydro-beta-carboline in food plants could be quantitatively determined in the concentrations of 0.1-50.0 ng/mL of homogenate. The recovery was almost 100% from all the tested plants, and the relative standard deviation was less than 8.4% within and between assays. Application of the proposed method has revealed that tomato, banana, pineapple, kiwi, plum and prune contain both tetrahydro-beta-carbolines at ng to mu g/g levels, and that their contents depend on the ripening process.

引用

页码：297 / 301

页数：5

共 27 条

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