CLONING AND OVEREXPRESSION OF RHAMNOSE ISOMERASE AND FUCOSE ISOMERASE

被引：14

作者：

GARCIAJUNCEDA, E ^{[1
]}

SHEN, GJ ^{[1
]}

ALAJARIN, R ^{[1
]}

WONG, CH ^{[1
]}

机构：

[1] Scripps Res Inst, DEPT CHEM, LA JOLLA, CA 92037 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1995年 / 3卷 / 10期

关键词：

D O I：

10.1016/0968-0896(95)00119-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhamnose isomerase and fucose isomerase were overexpressed in E coli, purified and characterized. The rhamnose isomerase gene was ligated to the restriction sites of PstI and Hind III of vector pTrcHis and the fucose isomerase gene was ligated to the EcoRI and PstI sites of vector pKK223-3 for overexpression of the enzymes in E. coli XL1-Blue MRF. Approximately 16,500 U of active fucose isomerase and 2400 U of rhamnose isomerase can be obtained per liter of culture from these expression systems.

引用

页码：1349 / 1355

页数：7

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