TRANSCRIPTIONALLY DRIVEN CRUCIFORM FORMATION INVIVO

被引：103
作者：
DAYN, A
MALKHOSYAN, S
MIRKIN, SM
机构：
[1] UNIV ILLINOIS,DEPT GENET,808 S WOOD ST,CHICAGO,IL 60612
[2] CALIF INST BIOL RES,LA JOLLA,CA 92037
来源：
NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 22期
关键词：
D O I：
10.1093/nar/20.22.5991
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We studied the formation of d(A-T)n cruciforms in E.coli cells by probing intracellular plasmid DNA with chloroacetaldehyde followed by fine analysis of modified DNA bases. d(A-T)16 sequences were inserted into specifically designed plasmids either upstream of a single trc promoter, or between two divergent trc promoters. We found that in both cases, induction of transcription by IPTG leads to the transition of the d(A-T)16 stretch into a cruciform state. In the case of two divergent promoters, we observed cruciform formation even without IPTG. Enhanced cruciform formation correlates with the elevation in promoter activity as defined by the opening of the promoter at the -10 to +2 positions. We conclude that transcriptionally driven negative supercoiling provokes cruciform formation in vivo.
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收藏
页码：5991 / 5997
页数：7
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