A MONOCLONAL-ANTIBODY RECOGNIZING AN EPITOPE ASSOCIATED WITH PIG INTERLEUKIN-2 RECEPTORS

被引：84
作者：
BAILEY, M
STEVENS, K
BLAND, PW
STOKES, CR
机构：
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 153卷 / 1-2期
关键词：
INTERLEUKIN-2; RECEPTOR; MONOCLONAL ANTIBODY; (PIG);
D O I：
10.1016/0022-1759(92)90309-H
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A monoclonal antibody is described which recognises an epitope associated with a receptor for interleukin-2 (IL-2) on pig lymphocytes. The monoclonal antibody inhibits high affinity binding of radiolabelled recombinant human IL-2 (rhIL-2) by pig lymphoblasts and also non-competitively inhibits both pig-TCGF and rhIL-2 maintained proliferation. By flow cytometry the antigen is apparently not present on freshly isolated blood lymphocytes but is detectable on small cells between 6 and 12 h after activation and on large cells by 24-h. These findings are comparable with those obtained using monoclonal antibodies recognising the 55 kDa a chain of the human and mouse IL-2 receptor (p55, TAC) expressed on activated cells in vivo and in vitro. However, the molecular weight of the porcine antigen is between 65 and 70 kDa.
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页码：85 / 91
页数：7
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