OLIGOCLONAL EXPANSION AND CD1 RECOGNITION BY HUMAN INTESTINAL INTRAEPITHELIAL LYMPHOCYTES

被引：235
作者：
BALK, SP
EBERT, EC
BLUMENTHAL, RL
MCDERMOTT, FV
WUCHERPFENNIG, KW
LANDAU, SB
BLUMBERG, RS
机构：
[1] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DIV GASTROENTEROL,MED RES BLDG,ROOM 013B,BOSTON,MA 02115
[2] UNIV MED & DENT NEW JERSEY,DIV EBERT GASTROENTEROL,NEW BRUNSWICK,NJ 08903
[3] HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DIV HEMATOL ONCOL,BOSTON,MA 02215
[4] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,CTR NEUROL DIS,BOSTON,MA 02115
来源：
SCIENCE | 1991年 / 253卷 / 5026期
关键词：
D O I：
10.1126/science.1716785
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha-beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.
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页码：1411 / 1415
页数：5
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