GENOTYPIC DIVERSITY AMONG BACILLUS-CEREUS AND BACILLUS-THURINGIENSIS STRAINS

被引：157

作者：

CARLSON, CR

CAUGANT, DA

KOLSTO, AB

机构：

[1] UNIV OSLO,CTR BIOTECHNOL,N-0316 OSLO 3,NORWAY

[2] UNIV OSLO,INST PHARM,N-0316 OSLO 3,NORWAY

[3] NATL INST PUBL HLTH,DEPT BACTERIOL,N-0462 OSLO,NORWAY

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 06期

关键词：

D O I：

10.1128/AEM.60.6.1719-1725.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Twenty-four strains of Bacillus cereus were analyzed by pulsed-field gel electrophoresis (PFGE) and compared with 12 Bacillus thuringiensis strains. In addition, the 36 strains were examined for variation in 15 chromosomal genes encoding enzymes (by multilocus enzyme electrophoresis [MEE]). The genome of each strain had a distinct NotI restriction enzyme digestion profile by PFGE, and the 36 strains could be assigned to 27 multilocus genotypes by MEE. However, neither PFGE nor MEE analysis could distinguish between the two species. Two of the B. cereus strains contained extrachromosomal DNA that hybridized to a cryIA insecticidal toxin probe, and seven strains contained DNA with homology to a Tn4430 transposon probe derived from B. thuringiensis. The results strongly indicate that B. cereus and B. thuringiensis should be regarded as one species.

引用

页码：1719 / 1725

页数：7

共 44 条

[1] INCIDENCE OF BACILLUS-CEREUS IN MILK AND SOME MILK-PRODUCTS [J].